Enzyme Kinetics and Mitochondrial Activity

This assignment explores the principles of enzyme kinetics by examining the activity of succinate dehydrogenase in isolated mitochondria. Students analyze experimental data on the reduction of DCIP (2,6-Dichlorophenolindophenol) as a measure of enzyme activity. They then calculate specific enzyme activities for both mitochondrial pellet and supernatant fractions, drawing conclusions about mitochondrial function based on these results. The assignment emphasizes data interpretation, critical thinking, and understanding the role of enzymes in cellular processes.