Design a Short Primer Sequence for PCR Amplification

The assignment content discusses the polymerase chain reaction (PCR), agarose gel electrophoresis, and CRISPR/Cas9 technology. PCR is a laboratory technique used to amplify specific DNA sequences by denaturing, annealing, and extending primers. Agarose gel electrophoresis is a method for separating and visualizing nucleic acids, where DNA fragments migrate through an agarose gel according to their size and are stained with ethidium bromide. CRISPR/Cas9 technology is a tool used for editing genes by introducing double-stranded breaks in the DNA sequence, which can be repaired by error-prone processes, resulting in insertions/deletions that may disrupt gene function.