An Overview of Microplate Reader Technology

Biomedical
sciences

Micro plate reader
▪ A micro plate reader is used for the measurement of the
interaction between different of a biological or a chemical
specimen in the well of a micro plate (Duellman et al.,
2015).
▪ Small micro wells present on the plate constitute a micro
plate.
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The working principle of micro plate reader
▪ The light signals emitted by the biochemical reaction of the
sample and the substrate present in the micro well are detected
by the micro plate reader.
▪ The type of sample and the concentration of the sample are the
major determinants of the light intensity.
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Recent development in micro plate technology
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• The recent advancement in the field of micro plate reader has
made detection of different diseases very easy.
• Types of micro plate readers
Multimode Micro plate reader
Luminescence Micro plate reader
Absorbance Micro plate reader
Fluorescence Micro plate Reader

Mechanism for the detection
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Types of ELISA Typical ELISA plate
Source :https://www.mybiosource.com/learn/ELISA

Diseases detection using micro plate reader
The Micro plate Readers have been used for the detection of many diseases such as
▪ Dengue: Fever occurs due to dengue virus, detection is carried out based on the
antigen related to the virus.
▪ The Virus mode of transmittance from one person to other is through mosquitoes.
▪ Major problem for tropical and sub tropical region.
▪ The detection is carried out using immunoglobulin M(IgM) and IgG antibody
produced by the immunes system in the response of dengue viruses on a ELISA
plate reader.
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References
▪ Berg, et al., 2015. Cellphone-based hand-held microplate reader for point-of-
care testing of enzyme-linked immunosorbent assays. ACS nano , 9(8),
pp.7857-66.
▪ Cannon, T.M., Shah, A.T., Walsh, A.J. & Skala, M.C., 2015. High-throughput
measurements of the optical redox ratio using a commercial microplate
reader. Journal of biomedical optics, 20(1), p.010503.
▪ Dong, S. et al., 2012. Cytotoxicity and cellular uptake of cellulose
nanocrystals. Nano Life, 2(03), p.1241006.
▪ Duellman, T., Burnett, & Yang, J., 2015. Quantitation of secreted proteins using
mCherry fusion constructs and a fluorescent microplate reader. Analytical
biochemistry, 473, pp.34-40.
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References
▪ Hanasand, M. et al., 2012. Improved detection of advanced oxidation protein products in plasma. Clinica chimica , 413(9-10), pp.
901-906.
▪ Hara, M. et al., 2012. "Urinary podocalyxin is an early marker for podocyte injury in patients with diabetes: establishment of a
highly sensitive ELISA to detect urinary podocalyxin. Diabetologia, 55(11), pp.2913-19.
▪ Jain, S., Chattopadhyay, , Jackeray, & Singh, H., 2012. Highly sensitive detection of Salmonella typhi using surface aminated
polycarbonate membrane enhanced-ELISA. Biosensors and Bioelectronics, 31(1), pp.37-43.
▪ MarketWatch, 2019. Microplate Reader Market 2019 Share Growing Rapidly with Recent Trends, Development, Revenue, Demand
and Forecast to 2025. [Online] Available at:
https://www.marketwatch.com/press-release/microplate-reader-market-2019-share-growing-rapidly-with-recent-trends-developme
nt-revenue-demand-and-forecast-to-2025-says-researchreportsworldcom-2019-11-25
[Accessed 21 March 2020].
▪ Thiha, A. & Ibrahim, F., 2915. A colorimetric enzyme-linked immunosorbent assay (ELISA) detection platform for a point-of-care
dengue detection system on a lab-on-compact-disc. Sensors, 15(5), pp.11431-41
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