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Aptamer Biosensor for Measuring Protein : Assignment

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Added on  2020-01-21

Aptamer Biosensor for Measuring Protein : Assignment

   Added on 2020-01-21

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Introduction(Aptamer Biosensor)Measuring the level of protein using conventional methods is very expensive and time consuming. There is a need of developing a method or an equipment that is capable of measuring or quantifying them and have certain advantages such as less complex, cheap and easy to use. Aptamers based Biosensor provide solution to this complex problem. These are special RNA or ssDNA based oligonucleotides. They have special structure that is capable of favouring bioregion part of biosensor. These biosensors have been developed to target all types as sizes of protein. These Aptamers based sensors provide cheap, reliable, less complex way of detecting proteins and hence play a vital role in health sector. BiosensorBiosensors are sometimes also referred to as bioelectronics, these are sensors which are made up of biological materials like cell receptors, enzymes and antibodies to detect analyte and used with the combination of physiochemical detectors [1] . Figure 1Source: Biosensors Young H. Lee and Raj Mutharasan, Department of ChemicalEngineering, Drexel University (2014)Physiochemical detectors are basically a type of transducer that converts one type ofsignal into another signal. This process may take place by physiochemical, electrolyte or piezo electrically. Existing technologies for protein identificationOther technologies that are capable of measuring protein is protein mass spectrometry. This is an expensive technique as compared to Aptamer biosensor. First the protein is ionized by using either of two techniques:Electrospray ionizationMatrix assisted laser ionizationAfter ionization they are passed through spectrometer in which different peaks are obtained that correspond to different mass and types of protein. These peaks are
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