Assignmment on Biochemistry

Running head: BIOCHEMISTRY 1
Biochemistry
Name
Date of Submission

BIOCHEMISTRY 2
Instructional Activity
Name
Date of Submission

BIOCHEMISTRY 3
1. D1S80 copies?
The size of a human genome is about 6billion bps while the average size of nucleotide bp
is 660 g/mol.
DNA from a single cell is 6billion X 660g
=3.96 pg
Thus there are two target copies of the gene in every 3.96pg of DNA. In theory, the in
human genome, about 25 to 100 ng of DNA is used for PCR. In 25ng, the copies of
DNA are 25000pg/3.960 multiply by 2 = 12625 copies (in 25ml reaction volume)
2. Safety
Wearing gloves
The pipette tips were changed after every sample addition to a PCR tube
No student was allowed to set up anyone else’s PCR
Each student set up a PCR reaction that contained their individual isolated DNA
Small reaction volumes were used
3. Method
For the 25 microliter reaction:
Mgcl2 =1.0 microliters
dNTPs= 4.0 microliters
buffer (Tris)=5 microliters
KCL= 0.25 microliters
Gelatin= 5.0 microliters