Bioinformatics Workflow: NCBI and BLAST Sequence Analysis

This assignment details a bioinformatics workflow for analyzing the GAPDH gene sequence of the plant species *Cymbidium faberi*. The workflow begins by retrieving the gene sequence from the NCBI database, followed by downloading it in FASTA and GeneBank formats. The assignment then guides the user through accessing protein information and identifying the protein domain using the NCBI database. Subsequently, the workflow involves running a BLAST search to identify similar sequences and selecting five closely related sequences based on E-value, query coverage, and percentage identity. The amino acid sequences of these selected sequences are then obtained from NCBI. Finally, the assignment concludes with the construction of a phylogenetic tree using Clustal Omega, based on the sequence alignment of the selected sequences against the assigned sequence.