The assignment is based on a biostatistics course and consists of five questions. Question 1 uses the binomial test to determine if there is a significant difference in the proportion of females and males, with the result showing no significant difference. Question 2 uses the Wilcoxon rank sum test to determine if there is a significant difference in MVPA hours between males and females, with the result showing no significant difference. Question 3 tests whether the concentration of PAH has decreased from introduction of new laws using a paired t-test. Question 4 uses chi-squared test to determine if sex and license status are independent of each other, with the result showing that they are independent. Question 5 calculates the required sample size for various studies based on desired standard deviation and margin of error.