Biotechnology Assignment Sample
Added on - 29 Apr 2020
Biotechnology2Question 4 (Propose a solution)The yield coefficient of production of bioethanol is obtained from the formula belowThe ethanol yield coefficient =Maximumethanolconcentration(grammesperlitre)Utilizedglucose(grammesperlitre)Waste products of pineapple peels can be employed to help produce a maximum yieldcoefficient of bioethanol. And this protocol can be developed in the laboratory to assess ethanoland glucose yield from pineapple peels which are considered as a waste. The consequence of theanalyzed quality of wholemeal peels of pineapple from the waste substances and the amount ofsaccharification of the amount of produced glucose was predicted[ CITATION Ban13 \l 1033 ].Inthe production of bioethanol from pineapple can be done using the following materials;i.Pineapple: pineapple peels are majorly obtained from the pineapple edible juicewhich is in most cases thrown away as a waste.ii.Enzymes: There are two major enzymes employed during the hydrolysis of starch.Glucoamylase. This enzyme is normally got from Rhizopus and Aspergillusniger. For this enzyme works best at a temperature of a range 550-600Cά-amylase. Is got from Aspergillus oryzae and Bacillus Licheniformis. Theenzyme is applied to degrade the starch from the wheat substance and it isstable at a temperature of 900CYeast. This acts as enzyme works best at a 350C to enable the fermentationoccurs.
Biotechnology3Experimentation.In the production of bioethanol is prepared through fermentation of the wheat since itcontains starch and sugar. This production takes the following five steps.Crushing of the pineapple to release the starch component.Filtering to remove the solid substances.Addition point where water, heat, and enzymes are introduced to enable the conversioninto fermentable sugar.Fermentation using the yeast. At this point, it is converted to Carbon (IV) oxide andbioethanol.Rectification and the distillation, at this point cleaning and concentration of the ethanol isdone by distillation and removing the co-product from the bioethanol[ CITATION DWI11 \l1033 ].The final step is the drying of the bioethanol which is also known as dehydration.From the below equation (i) which shows the fermentation equation during the process, we canobtain the amount of glucose used during the reaction;C6H12O6→ 2 C2H5OH + 2 CO2................................................... (i)Every molecule of glucose is then decomposed into two pyruvates, the process referred to asglycolysis. The process of glycolysis is expreesed by the following equation.C6H12O6+ 2 ADP + 2 Pi+ 2 NAD+→ 2 CH3COCOO−+ 2 ATP + 2 NADH + 2 H2O + 2 H+............ (ii)