Bradford Assay: A Safe and Effective Method for Protein Quantification

Running Head: BIOLOGY
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Biology

BIOLOGY
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Abstract:
In the year 1970s Mraion M. Bradford developed Bradford assay which has been identified as
the safe, accurate and most effective method for analysing the protein concentration in the
aqueous solution with the use of spectroscopic analytic process. Coomassie blue dye is used
in the assay for determining uncertain amount of protein. For the purpose of carrying out an
experiment, standard curve using BSA at the particular range of the scale from 0 to 1.0mg/ml
has been constructed, and later on the technique of the Bradford Assaying was performed.
The result obtained from the experiment determined the concentration of the unknown
sample, which was observed as Sample A, 0.77mg/ml and the sample B was observed as
0.37mg/ml.

BIOLOGY
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Contents
Introduction:...............................................................................................................................3
Materials and Methods:..............................................................................................................4
Results:.......................................................................................................................................5
Discussion:.................................................................................................................................7
References..................................................................................................................................9