Effect of Hydrogen Peroxide Concentration on Catalase Enzyme Reaction

Carrying Out a Practical
Investigation (Biology)

TABLE OF CONTENTS
Aim..............................................................................................................................................3
Rationale......................................................................................................................................3
Methodology................................................................................................................................3
Approach......................................................................................................................................4
Philosophy...................................................................................................................................5
Research Design..........................................................................................................................5
Data Collection............................................................................................................................5
Data analysis................................................................................................................................7
Results and Findings....................................................................................................................8
CONCLUSION............................................................................................................................9
Limitations...................................................................................................................................9
REFERENCES..............................................................................................................................10

Title: To show the effect of Hydrogen Peroxide concentration on the rate of catalase enzyme
reaction.
Aim
To analyse the effect of Hydrogen Peroxide concentration on the rate of reaction of catalase
enzyme.
Rationale
The main reason of doing the research is to analyse the effect which hydrogen peroxide
concentration has on the reaction rate of catalase enzyme. This reaction is used in majority of the
reactions which take place in chemistry labs. This is why it must to analyse this impact.
Hydrogen peroxide is the most important chemical which act as mild antiseptic which can help
in preventing infection of minor cuts, burns or scrapes (Sindi and et.al., 2019). Catalase enzyme
is also a very important enzyme which is found in living organisms which are exposed to oxygen
such as plants, animals, bacteria etc. This decomposes the hydrogen peroxide into oxygen and
water and helps in protecting cell from oxidative damage by reaction with the oxygen species
(ROS).
Methodology
The methodology chosen in this research is the quantitative type of research which is
used to investigate about the effect of Hydrogen Peroxide concentration on the rate of catalase
enzyme reaction. Enzymes are most essential things for the survival of human beings. These are
considered as the proteins which help in transforming the chemicals in the body which functions
as a catalyst. Catalyst helps in getting the reactions happening faster by increasing the rate of the
reaction. In every reaction, there is a need of minimum amount of energy which is known as
activation energy (Hu and et.al., 2017). This was observed that lower the activation energy is the
faster the reaction takes place. On the other hand, if the activation energy is too high then the
reaction does not occur at all.
Enzymes have the ability for lowering the activation energy of chemical reaction through
interaction with the chemicals involved in the reaction. There are various enzymes among which
catalase are a very common enzyme which is present in all the organisms which are exposed to
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oxygen. The main aim of the catalase enzyme in the living cells is protecting the cells from the
oxidative damage which basically occurs when the molecules or the cells come in contact with
the other oxidative compounds. This is not a man made process instead a result of natural
process or reaction which happens inside the cells of human beings (Litosseliti, 2018). There can
be involvement of the by-products in the reactions such as hydrogen peroxide which is
considered to be harmful for the body. This can be similar to the ways in which by-product of a
nice bonfire can become unwanted smoke which leads to cough or stings to the eyes. In order to
prevent such damage, the catalase enzyme helps to get rid of these compounds when the
hydrogen peroxide (H2O2) is broken down into harmless water and oxygen.
The practical methods are mostly used in this research to achieve the objectives. The
methodology which was applied for gaining the quantitative information in this research is the
practical experimentation which is done and the results were found. This is why; the research
was based on the quantitative type of information. This methodology can be appropriate in order
to gain significant findings and outcomes. This is chosen because there is no need of the
evidence because the practical is performed which can help in providing the evidence. The
experiment was done by 4 groups in which they performed the experiment with different
concentrations of hydrogen peroxide (Atmowardoyo, 2018). This research aim can only be
achieved by making use of quantitative information which can be obtained by practical which is
performed by the groups.
Approach
This is the section of research which decides that whether any theory needs to be tested or
any theory needs to be generated. On the same basis, there are two main types of approaches
which are inductive and deductive. Inductive approach deals in creating and developing theory
while on the other hand the deductive approach lays emphasis on the testing of the theory. In this
theory no theory can be generated after getting the outcomes and conclusions instead hypothesis
needs to be checked and tested. Catalase enzyme is considered to be acting as catalysing enzyme
in decomposing substance into another (Cypress, 2018). Catalase enzyme is responsible for
decomposing or breaking down hydrogen peroxide into oxygen and water. Based on this theory,
research will be done, this is how deductive approach will be used in this research. This will help
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