CHROMATOGRAPHY EXPERIMENT2Chromatography Experiment.SolventObservationsEthanolOn addition of ethanol as a solvent approximately 13 ml of methylene blue dye drained out of the column shown by the dark blue eluent. During this time the fluorescein dye only moved very slightly. WaterWhen water was added as a solvent caused it caused the migration a greenish yellow liquid which is most likely to be the fluorescein dye. The eluent collected was around 14 ml. Observations/Results:Part A: Separation of a dye mixture by solid-liquid (column) chromatography.Table 1: Separation of a dye mixture by solid-liquid (column) chromatographyPart B: Separation of plant pigments by paper chromatography Table 2: Rf values of the different pigments in spinach.Identify the pigments and their Rf values.Distance travelled by solvent front: 10 cmRf = Distance travelled between the starting point and pigment lineDistance between the starting point and solvent front Pigment Distance Travelled by Rf Value
End of preview
Want to access all the pages? Upload your documents or become a member.
Related Documents
Laboratory Report on Chromatographic Techniqueslg...
|7
|1881
|24
Betalains and their Applications in Food Industrylg...