Bacterial Clonal Diagnostics for Evidence-Based Empiric Antibiotic Selection

Running head: MEDICAL MICROBIOLOGY
MEDICAL MICROBIOLOGY
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1MEDICAL MICROBIOLOGY
Introduction
Antimicrobial resistance has become one of the major issues of healthcare system as
there are no such approaches to determine the antimicrobial susceptibility. Hence, requirement of
different speed approaches for determination of susceptibility becomes very crucial. In this
paper, the researchers assessed the clonal diagnostic approach for the empirical antibiotic
selection. For this purpose, it uses an observational cohort study and used the quantitative PCR
tests to determine the clonal groups of 50 Escherichia Coli and within which they significantly
reduced the risk of antibiotic mismatch.
Methods
There are several material and methods that were used for the determination of antibiotic
cloning and susceptibility and these were prospective clonal diagnostic study, 7- SNP test, local
reference set, multinational reference set, urine culture and isolate testing and statistical analysis
and finally the calculations related to prescription mismatch rates. Based on the data that was
derived from the different tests, the data had been analyzed using the statistical analysis. Besides
in this process, while determining the methodology 20, 10 and 30% resistance threshold was
allowed.
Results
From the 750 urinalysis samples, it was seen that 274 people contain E coli which was
determined from the 7-SNP test. Between the 267 samples that were discovered to have E. coli
by both the 7-SNP test and culture, in 260 (97%) the clonotype determined directly in urine
corresponded with that determined by single-colony testing. The study isolates encompassed a