Comparison of Microscopy Techniques

Running head: COMPARISON OF MICROSCOPY TECHNIQUES
Investigating biomaterials: structure, topography, morphology and composition using
microscopy techniques
Name of the Student
Name of the University
Author Note

1COMPARISON OF MICROSCOPY TECHNIQUES
Table of Content
Introduction......................................................................................................................................2
Types of Microscopy.......................................................................................................................3
Implantation and use of microscopy................................................................................................4
Utilisation and significance of optical or light microscopy.............................................................5
Utilisation and significance of Electron microscopy.......................................................................7
Selection of appropriate method....................................................................................................10
Conclusion.....................................................................................................................................11
References......................................................................................................................................13

2COMPARISON OF MICROSCOPY TECHNIQUES
Introduction
Microscopy is referred to the technological field of using microscope for viewing objects
as well as areas of objects that cannot be seen with the help of naked eyes. The three most well
known branches of microscopy include optical microscopy, electron microscopy and scanning
probe microscopy. Another emerging field of microscopy includes X ray microscopy. When it
comes to optical microscopy and electron microscopy, it includes reflection, diffraction as well
as refraction of electromagnetic radiation that interacts with the specimen along with the
collection of the scattered radiation as well as another signal for creating an image (Gigan 2017).
When it comes to scanning probe microscopy, it includes the contact of a scanning probe with
the object of interest’s surface. The development of microscopy is a revolutionary act in biology.
This invention gave rise to the field of histology and hence remains a highly crucial technique in
the life of physics. When it comes to X ray microscopy, it is both three dimensional as well as
non destructive that allows repeated imaging of the same sample for 4D studies as well as in Situ
along with providing the ability for studying the sample before sacrificing the same to higher
resolution techniques. A 3D X ray microscope is found to use the techniques of computing
tomography, rotating the sample 360 as well as reconstructing the images (Peng et al. 2015). In
this report, types of microscopy along with the use of microscopy for implantation will be
discussed. Moreover, evaluation of the optical microscopy as well as electron microscopy will
also be conducted in this report. Along with this, the advantages of the electron microscopy over
the optical microscopy will also be discussed in this report. The chief aim of this report is to
appraise the usage, advantage as well as limitations of the optical and electron microscopy
techniques applied to implanted medical devices.

3COMPARISON OF MICROSCOPY TECHNIQUES
Types of Microscopy
When it comes to the healthcare industry, generally three types of microscopy is used in
the mentioned industry, namely, optical microscopy, scanning probe microscopy and electron
microscopy.
Optical Microscopes:
This type of microscopy involves microscopes that bounce light of the object that is being
viewed with the help of the same. Diffraction, refection as well as refraction are used by these
microscopes for magnifying the image until it is seen. The optical microscope is comprised of
one or more lenses, after placing the object near the focal point, the combination of produces a
significantly enlarged image of the specimen that.
Electron Microscopes:
One of the most significant alternatives of the optical microscope includes the electron
microscope. For generating images a beam of electron is used in the electron microscope uses
instead of light. The range of wavelength visible light used in the optical microscope is 4000
Ampere to 7800 Ampere while the wavelength for electronic microscope is 60 to 80 KV
electrons and the wavelength is only 0.05 Ampere (Nienhaus and Nienhaus 2016).
Scanning probe microscope
The scanning probe microscope was created as a practical instrument with the help of the
quantum tunnelling theory. The probe approaches the surface closely enough that electrons can
flow continuously between the sample as well as the electron creating a current from surface to