Competition Dialysis: A Tool for Ligand Discovery in Nucleic Acids

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Explain competition dialysis;
Competition dialysis refers to a new tool that is used in the discovery of the ligands that are
known to bind to the nucleic acids. These binding are restricted to those nucleic acids with
structural selective activity (Kumar 2012). In this particular method, a common test solution is
used as a reference standard point when a particular type of the structure of nucleic acid is to be
dialysed.After the process of equilibration is achieved,absorbence or fluorescence measurements
are used in the determination of the of the amount of ligand that is bound to each structure.
Sometimes the bounding is on the sequence.
Figure 1.Sample results of competition dialysis extracted from (Sannasi and Santoso 2016).
How can it be used?
The well-known process of dialysis involves flow of one or more solutes by diffusion through a
semi-permeable membrane. Those molecules that are smaller in size as compared to the size of
the pores of the membrane will definitely pass through. Inside the membrane are retained
particles of larger sizes. The process of dialysis is used in the basic science of impurity removal