Assignment on Dornase Alpha PDF

Pet Protein Study: Sequence and Purification for DORNASE ALFA You will be given the name of a protein of industrial/medical importance. You will need to select a specific species variant that is used in a medical or industrial application. Find out about: 1. The primary structure (amino acid sequence) of your Pet Protein. Prepare your own figure (not a journal extract) of the primary sequence, clearly showing the numbering relevant to the mature sequence. Outline and label any sub-regions or domain units important in this protein. Indicate on this figure the disulfide connections (if any), as well as any amino acids known to be glycosylated. 2. The physico-chemical properties (pI, MW value) of the protein that are important considerations for a purification protocol. Use the tools in ExPASy to analyse your sequence. Check you have the mature sequence of your protein (i.e. remove any signal peptide) If a glycoprotein, detail the full chemical nature of the carbohydrate por