Assignment on Dornase Alpha PDF

Running head: BIOLOGY
DORNASE ALPHA
Name of the Student
Name of the University
Author Note

BIOLOGY1
Table of Contents
Introduction................................................................................................................................2
1. The primary structure (amino acid sequence):.......................................................................2
2. The physico-chemical properties (pI, MW value) of the protein that are important
considerations for a purification protocol:.................................................................................0
Predicted proteins..................................................................................................0
ProtScale..................................................................................................................................1
User-provided sequence:...................................................................................................1
3. How the protein was initially purified from its natural source when it was first discovered:3
4. How is the same protein now prepared for an industrial use, or in recombinant form:.........4
Bibliography...............................................................................................................................6

BIOLOGY2
Introduction
Dornase alfa is a highly purified solution containing the recombinant DNase I protein of
human beings. This is an enzyme solution which is responsible for the selective cleavage of
DNA. This enzyme has been found to hydrolyse the DNA which has been found to be present
in the sputum/mucus of the patients suffering from cystic fibrosis and has been found to
reduce viscosity in lungs in order to facilitate airway clearance. The trade name of this
enzyme is Pulmozyme. This paper will discuss the primary structure, physicochemical
properties and purification processes of Dornase alfa.
1. The primary structure (amino acid sequence):
10 20 30 40 50
MRGMKLLGAL LALAALLQGA VSLKIAAFNI QTFGETKMSN ATLVSYIVQI
60 70 80 90 100
LSRYDIALVQ EVRDSHLTAV GKLLDNLNQD APDTYHYVVS EPLGRNSYKE
110 120 130 140 150
RYLFVYRPDQ VSAVDSYYYD DGCEPCGNDT FNREPAIVRF FSRFTEVREF
160 170 180 190 200
AIVPLHAAPG DAVAEIDALY DVYLDVQEKW GLEDVMLMGD FNAGCSYVRP
210 220 230 240 250
SQWSSIRLWT SPTFQWLIPD SADTTATPTH CAYDRIVVAG MLLRGAVVPD
260 270 280
SALPFNFQAA YGLSDQLAQA ISDHYPVEVM LK
Signal peptide sequence= Positions 1 to 22 [Yellow]
Mature chain sequence= Positions 23 to 282 [Green]
Glycosylation= N-linked glycosylation of 40th residue the protein (Asparagine) [Red] and N-
linked glycosylation of 128th residue the protein (Asparagine) [Red].
Disulphide bond= 123rd to 136th residue and 195th to 231st residue. The latter one is
responsible for enzyme activities

BIOLOGY3