E.coli Pathogens Biofilms In Urinary Tract Infections

E.coli Pathogens Biofilms In
Urinary Tract infections

Table of Contents
INTRODUCTION...........................................................................................................................1
MAIN BODY...................................................................................................................................1
Identify a comprehensive range of relevant literature to the topic.........................................1
Link the findings of your literature search to current questions and new research in the field to
identify a sub-topic to focus on for your review....................................................................2
Critically analyse the current research literature in your chosen sub-topic............................3
Evaluate the contribution of the literature you discuss to advancing the field of research....5
Assess the quality and reliability of the data in the literature.................................................6
CONCLUSION................................................................................................................................6
REFERENCES................................................................................................................................7

INTRODUCTION
Escherichia coli are known to be a gram negative bacterium and present in human gut. Biofilms
are communities of microorganisms and their functional products assisting bacteria to attach to
uroepithelial cells. E. coli refers to a bacterium which is responsible for having around 70% to
95% of urinary tract infection that may occur in human beings (Sevanan, Pongiya and
Peedikavil, 2011). It develops different types of infection which are commonly known as UTISs
(urinary tract infections). In context of this assignment, it is based on the E. coli pathogens
biofilms in urinary tract infections with the help of reviewing an article. This essay will focus on
literature of selected topic and identify the sub topics to conduct review. It will also include the
analyse current research literature and evaluate the contribution of the same. The quality and
reliability of the data mentioned on literature is given below.
MAIN BODY
Identify a comprehensive range of relevant literature to the topic
E. coli is a pathogen that is accountable for present in gut of human beings due to which various
types of urinary tract infections takes place. The selected article is based on the topic of
‘Detection of biofilm among uropathogenic Escherichia coli and its correlation with antibiotic
resistance pattern’. It is observed that UTIs are known as serious health issue in regards to
antibiotic resistance for which biofilms preparation being a prime regarding this problem. The
given investigation focus on the research aim of performing in vitro detection of biofilms among
E. coli strains isolated from urine as well as for correlating their susceptibility pattern with
biofilm formation (Saroj and et.al., 2012).
It has been evaluated that around 100 E. coli strains isolated from infected individuals facing
problem of urinary tract infections to carry out the study. The criterion if identifying the bacterial
has been performed with the help of Gram staining, biochemical tests and colony morphology to
determine E. coli. However, biofilm got detected through several methods such as Congo red
agar (CRA) method, tube method (TM) and tissue culture plate (TCP) method. The procedure of
Kirby-Bauer disc diffusion was carried out on Muller-Hinton agar plate in order to perform
antimicrobial sensitivity testing.
This article has several findings such as 49 (49%) and 51 (51%) were belongs to catheterised and
non-catheterised patients out of collected sample of 100 E. coli strains. It consist the positive
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