Effect of Hydrogen Peroxide on Chicken Liver

Added on - 11 Jan 2021

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LAB REPORT
TABLE OF CONTENTSTITLE.............................................................................................................................................1RATIONALE...................................................................................................................................1RESEARCH QUESTIONS.............................................................................................................1ORIGINAL EXPERIMENT............................................................................................................2MODIFICATIONS..........................................................................................................................2MANAGEMENT OF RISKS..........................................................................................................2IDENTIFIED VARIABLES............................................................................................................3QUALITATIVE DATA...................................................................................................................3QUANTITATIVE DATA................................................................................................................4PROCESSING DATA.....................................................................................................................5ANALYSING DATA......................................................................................................................7LIMITATIONS................................................................................................................................7ERROR CALCULATIONS............................................................................................................8IMPROVEMENTS..........................................................................................................................8CONCLUSION................................................................................................................................8REFERENCES..............................................................................................................................10
TITLETo determine the effect of hydrogen peroxide on chicken liverRATIONALEThis part includes an understanding to know about the effect of any substance whileundergoing a chemical reaction. Here role of catalase, hydrogen peroxide, factors affecting thereaction rate and its impact. A chemical reaction is defined as the arrangement of molecularstructure to bring the chemical changes by transforming the reactants into products. It is ofvarious types including single displacement, decomposition displacement and direct combustion.Next is the reaction rate which is defined as the speed of the chemical reaction and mostlyexpressed in amount per unit volume(Gong and et.al., 2019). This is measured when tworeactant molecules and is dependent on factors like temperature, reactant concentration, surfacearea and reactants states.In addition to the above, catalase acts as the driving force which is an enzyme and leadsto the decomposition of hydrogen peroxide into oxygen and water. It helps in preventing thedamages done to the cell oxidative and gives a detailed view of atomic and electronic aspects.Moreover, hydrogen peroxide is the colourless liquid element and is considered ashighlyreactive chemical containing hydrogen and oxygen (Gulyás and et.al., 2017). The emphasis is onunderstanding the key principles of collision theory and knowing the direct proportional to thetotal number of collision between the reactant molecules. This shed light on understanding theeffect of catalase on the rate of reaction and the increment and decrement values.This is based on understanding the role ofhydrogen peroxide percentage which has beendemonstrated by an increase number as compared to the amount of gas. Moreover, there hasbeen used of three different types of percentages of hydrogen peroxide will be used to determinewhich has the most effect on the catalase and produces the most amount of gas.RESEARCH QUESTIONS1.How does an increase in the percentage amount of hydrogen peroxide increase thereaction rate of a chemical reaction?2.Does an increase in the percentage amount of catalase increase the rate of reaction with1.5% of hydrogen peroxide?1
ORIGINAL EXPERIMENTThe aim of the original experiment was to determine the effect of hydrogen peroxide onchicken liver. By doing this experiment the catalase in the live broke down the hydrogenperoxide into water and oxygen. This was shown by the bubbles in the top of the test tube. Theliver was cut into 3 equally sized pieces, then the 3 equal pieces were slit into 6. 2 pieces werekept normal, another 2 pieces were cut up again into smaller pieces and the last 2 were crushedup. All 6 pieces were placed into 6 test-tubes. 1 of each type were placed into heated water andthe other 3 were left in room temperature. This experiment was texting the surface area of theliver. Due to results shown the two pieces that were crushed up had produced the most amount ofgas.MODIFICATIONSThe experimental methodology was refined to only modifying 1 variable to the originalexperiment. The concentration of the catalase was the independent variable being changed. Thecatalase had been split into 4 different percentages, these including; 6.25%, 12.5%, 25% and50%. The gas pressure sensor was used to measure the amount of gas produced in each reaction,using the percentage of catalase on 1.5% of hydrogen peroxide.The experiment set up was presented with using 4 test-tube racks each containing 5 test-tubesand the percentage catalase used for each rack. The gas pressure sensor was connected to alaptop which was connected to the data logger, all the data was represented by using the appVernier graphical analysis on a laptop.MANAGEMENT OF RISKSThere is definite ethical issues in relation to the risk management while conducting anyresearch in order to maintain appropriate disposal. This includes lack of technical expertise orlack of vigilance while performing the experiments by the scholar which might lead toinaccuracies. Moreover, it would be unfit in obtaining the results under proper systems and alsoemphasize on the data protection and security issues (Zheng and et.al., 2018). Thus, it can beminimised by segregating the tasks into smaller parts and store it in one specific location to avoidany copying or stealing activity. The focus is on minimising the operational and strategic riskswhich are the most common types of risks. This is overcome by following the compliance in2
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