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Evidence Based Practice in Laboratory MedicineName of the StudentName of the UniversityAuthor’s Note
Evidence based practice is an extensively used decision making process applied invarious service provision fields and is supported by strong scientific evidence to improveclient outcome very efficiently. In this regard, medical science is a domain where theevidence based practice have been utilised in the field of nursing and medicine to improvehealth outcomes of patients (1). Evidence based practice primarily relies on coordinatedcontribution of several aspects that includes expert opinion, scientific evidence and client,patient or caregiver perspectives and priorities. It is person centred practice that takes intoconsideration the needs, values, interests and choices of individuals receiving care. Theessential steps followed are framing the clinical question relevant to the patient, findingrelevant scientific evidence, assessing the evidence, making comparison with the real lifescenario and finally making the clinical decision. It strongly discourages use of singleobservations and intuition to make clinical decisions. Incorporation the principles of evidencebased practices in the field of laboratory medicine various issues like sufficiency of testing,profit of the laboratory and eventual clinical outcomes of the tests (2). The fitness oflaboratory medicine in the care pathway, nature and quality of testing, appropriate testing,utility of testing and the method in which the tests can impact clinical actions, all of thesefactors can be evaluated by applying evidence based practice in laboratory medicine. Two detailed examples of evidence based practice in our placement laboratory havebeen provided herein. Collagen Mason’s Trichrome Stain is a differential staining procedureused to differentiate between collagen and smooth muscle tissues in tumours. Moreover, it isused to detect the increase in collagen fibres in case of diseases like cirrhosis. It is one of themost routine staining tests for liver and kidney tissues. As suggested by the name threedifferent stains viz. hematoxylin (purple nuclear stain), mix of xylidin and acid fuscin (redcytoplasmic stain) and aniline blue (light green collagen fibre stain) constitute Mason'strichrome stain (3). The staining principle depends on the permeability of the tissues to the
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