Experimental Study - Leukocyte Specific Integrins In Scar Healing

Added on -2020-02-19

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Running head: EXPERIMENTAL STUDY: INTEGRINS IN SCAR HEALINGExperimental study: Leukocyte specific integrins in scar healingName of the StudentName of the UniversityAuthor Note
1EXPERIMENTAL STUDY: INTEGRINS IN SCAR HEALINGExecutive summaryCell adhesion regulation during wound healing is important for cell migration, cell proliferationand protein formation. The cells with the extracellular matrix interact with the integrin receptorsthat in return provide signals into the nucleus to regulate the gene expression and behavior.Specific integrins are thought play a major role in re-epithelialization and tissue granulation.Thus, in this report we will propose an experimental design in order to review the role of somespecific integrins in wound healing through a scarless invivo study.
2EXPERIMENTAL STUDY: INTEGRINS IN SCAR HEALINGTable of ContentsIntroduction......................................................................................................................................3Methods...........................................................................................................................................3Experimental design........................................................................................................................6Animals........................................................................................................................................6In vivo scar healing experiment...................................................................................................6Wound healing rates determination.............................................................................................7Indirect Immunofluorescence Microscopy..................................................................................7Light Microscopy.........................................................................................................................7Western Blot Analysis.................................................................................................................8Conclusion.......................................................................................................................................8References......................................................................................................................................10
3EXPERIMENTAL STUDY: INTEGRINS IN SCAR HEALINGIntroduction Wounds are common symptoms in skin caused by surgery or traumas. The coordinatedprocess of wound healing restores functional barrier and epithelium integrity through bloodcoagulation, inflammation, in re-epithelialization caused by migration of keratinocytes, tissueformation by granulation and finally remodeling of the tissue. Integrins receptors play anessential role in all events of wound and scar healing by the forming and regulation cell adhesion(Weber et al., 2012). In this following report, we will design an experimental model in order todiscuss the role of specific function-blocking integrin antibodies in healing and reducing thescars formed by burns through scarless invivo mechanism. MethodsDuring the process of wound healing the, cell interaction occurs with ECM molecules inthe wound comprising the integrin receptors. Evidence has showed that integrins bind withvarious ECM molecules and alternately the various integrin heterodimers recognizes the ECMmolecules. Therefore, based on their specific overlapping and compensating functions, manyintegrin based knockout animals have displayed phenotypes of wound healing. Many extensiveinteractions are found in the different cells of wound healing. The major components that play akey role during the interactions are ECM proteins and integrins (Olczyk, Mencner &Komosinska-Vassev, 2014). The functions of some of the specific integrin antibodies that play avital role in wound healing are provided below:Table 1: Specific integrin functions in wound healingIntegrinExpressedLigandsCellularWound

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