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Fluorescence microscopytutorial and quiz 2016http://www.olympusfluoview.com/theory/index.htmlCBMS880/731/331
Fluorescence microscopy tutorial and quiz Provide answers to the following questions in consultation with the interactive tutorial atwww.olympusconfocal.com/java/index.html and other relevant material made available to you.Also look into http://www.invitrogen.com/site/us/en/home/brands/Molecular-Probes.html? andother similar websites to answer question 3. For some background, have a look athttp://micro.magnet.fsu.edu/primer/lightandcolor/fluoroexcitation.html.Your tools: -The FLUOVIEW 1000 system at MU has four lasers diodes:405 nm, 473 nm, 543 nm and 635nm -Always go for the maximum excitation and emission possible.-You will find many of the probes in the table under the headings “Conventional dyes”, “Alexadyes”, “Fluorescent proteins” and “Cyanine dyes”.DsRED is a fluorescent proteinDiOC6(3) is similar to FITC, a traditional dyeTO-PRO-3® is a cyanine dye (Cy5)SYBR® Green I is similar to Alexa 488Laser types:Start with looking at the Laser Scanning Confocal Microscope Simulator
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