This assignment delves into the principles and applications of polymerase chain reaction (PCR) and agarose gel electrophoresis for analyzing changes in HeLa cell DNA levels, specifically focusing on the viral E6 gene. It outlines experimental procedures involving four main reactions: a control using HeLa cells, treatments with ATO 2uM and 5uM concentrations, and a no template control. The document emphasizes the importance of PCR cycles for amplifying DNA sequences and discusses potential variations between expected results and experimental outcomes due to factors like human error, measurement inaccuracies, and sample purity.