The provided sequences are from influenza A and B viruses. The nucleotide BLAST search shows that Sequence A is from 1968 and region H3N2, while Sequence B is from 1950 and region H1N1. No restriction enzyme cuts within the genes, so BamH I, Pst I, or Xho I can be used for cloning. The translated protein sequences are named based on the region and year (e.g., '1968-H3N2-Protein'). There are 7 point mutations between the two nucleotide sequences, predicting 5 amino acid differences. The actual alignment shows 12 different amino acids. The predicted number is less than the actual number due to insertions or deletions in the protein sequence. Protein alignments have separate identity and similarity measures because they account for different types of changes (substitutions, insertions/deletions). Evolutionary pressures drive flu virus evolution. Vaccines may lose effectiveness as the season progresses because the virus evolves rapidly. The flu virus evolves rapidly due to its high mutation rate and genetic recombination.
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