The article discusses the qualitative and quantitative studies on hand hygiene protocol and ways to maintain it. It covers the background, method of studies, result of studies, ethical consideration, outcome comparison, and conclusion. The studies suggest that hand hygiene is the most priority care that should be taken by every healthcare professional to reduce the level of infection in healthcare units and improve the health of patients. The article recommends that every healthcare sector must follow the protocol for washing hands by communicating the concern to every health department by the means of posters and presentation.