ELISA (Enzyme-Linked ImmunoSorbent Assay) for the immunization of mice with EAE equivalent

Running head: HEALTHCARE
MEDICAL AND APPLIED IMMUNOLOGY
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HEALTHCARE1
Assay 1: ELISA (Enzyme-Linked ImmunoSorbent Assay)
In the experiment, autoimmune disease (EAE equivalent) was introduced in mice strain
by administering an immunogenic epitope (MOG). Then it was suspended in Freund’s complete
adjuvant (CFA) before the immunization process. After a period of 9 to 14 days of introducing
with pertussis toxin, the mice were found to develop EAE (Experimental autoimmune
encephalomyelitis). One week after this experiment was performed, the mice were then
introduced with an experimental drug. ELISA can be used to check T cell functioning in
response to the above-stated drug. ELISA can be used for this test because of the fact that T cell
functioning is to be studied here. After the introduction of the drug, if there is an increase in the
efficacy of T cell functioning, it can be detected by ELISA. Body fluid from the mice needs to be
isolated and ELISA is to be performed with it. T cell activation is responsible for the release of
cytokines, which can be properly readout with ELISA. The ELISA wells need to be coated with
antibodies. Now, if T cell is properly functioning in the body of the mice, then it is bound to
release cytokines (Giavridis et al. 2018). These cytokines will bind to the antibody in the
microtitre well and are detected with the help of enzyme-substrate reaction. Thus, it can be stated
that if T cell functioning has increased in the mice after the introduction of the new drug, then
the cytokines will be detected in ELISA. Thus, it can be stated that ELISA can be used to assay
the functioning enhancement or deterioration of T cell after the introduction of a new drug. The
presence of adjuvant is also responsible for a slow release of antigen and thus makes the
detection process more accurate (Zhang et al. 2018). Various studies have been identified which
showed that ELISA can be used for T cell functioning in vitro assay procedures (Maj et al.
2017). Therefore, ELISA was chosen for the in vitro assay of T cell functioning of mice after the
introduction of a new drug for the prevention of EAE