Immunology Practical Assessment: FACS Analysis, Semester 1, 2018

This document provides a comprehensive solution to an immunology practical assessment focusing on FACS (Fluorescence-Activated Cell Sorting) analysis. The assessment includes detailed answers to questions regarding the identification of cell populations (neutrophils, lymphocytes), the significance of CD3 staining in T cells, and the information derived from comparing CD3 and CD4 staining data. It explores the relationship between T helper cells and immune responses, the prevalence of NK cells versus B cells in blood samples, and the identification of cells in Panel E (Th1 and Th2 cytokines). The solution also summarizes a CD8 vs. CD3 dot plot and explains the importance of isotype controls in flow cytometry, supported by relevant references. The assignment adheres to the provided word count and referencing guidelines.