Renal Function Lab Write-up Template

Intermediate Human Physiology (BY266)
Renal function lab class write-up template
Student No:

Materials and Methods (250 words maximum)
The aim of the following practical was to evaluate the diuretic effects administered
due to the ingestion of only orange juice (1 litre) or alcohol (25 ml, 40% vodka)
and furosemide (40 mg) across Bioscience students studying in 2nd year. Hence,
at the beginning of the experiment, the participants were instructed to empty their
bladders and perform experimental steps pertaining to measurement of volume of
urine and evaluation of the content of sodium ions (Na+) and Potassium ions (K+)
with the aid of a flame photometer. The participants were divided into three
groups, A, B and C, of which, Group A participants were asked to ingest 1000 ml
of squash, Group B was asked to ingest 1000 ml of squash along with 25ml of 65°
proof vodka and Group C was asked to ingest 1000 ml of squash along with 40mg
tablet of furesemide.
For measurement of urine volume, participants were asked to empty their bladder
in a jug of 1liter and measure the volume produced with the aid of a measuring
cylinder of 100 ml.
For measurement of potassium and sodium content of urine using a flame
photometer, participants were asked to dilute their urine volume using the formula
C1 x V1 = C2 x V2 (where, C1= undiluted urine concentration, C2 = concentration
of urine after a 1000 fold dilution, V1 = volume for dilution: 100 μl, V2 = 100 ml).
After the dilution, the participants were asked to aspirate the diluted samples of
urine in the flame photometers and note down the relevant readings for potassium
and sodium ions in the urine.