Introduction to Biochemistry : Assignment
8 Pages1432 Words131 Views
Added on 2021-04-24
Introduction to Biochemistry : Assignment
Added on 2021-04-24
ShareRelated Documents
Student No./10001Experiment: Glycoside Beta DrumminAssignment (type)Date1000 wordsStudent’s NameInstitutional Affiliation
Student No./10002Experiment: Glycoside Beta Drummin1) According to the Embden-Meyerhof pathway, the pentose cycle involves the conversion of glucose molecules (and fructose molecules) to CO2. Glycolysis involves the conversion of glucose to produce ATP. 3 pentose phosphates elements are converted to form fructose 6-phosphate and triose phosphate 1. The 14C are used directly while the fructose 6-P is not broken down to Glucose. As such, the 14C is used up to complete for the pentose phosphate cycle due to randomized of 14C metabolism use in the pentose cycle or glycolysis pathway. Insome cases, when the fructose-6-phosphate is equal or in equilibrium with glucose-6-phosphate, fructose-6-phosphate can be reversed. However, even when the process is reversed in glycolysis the use of 14C from the substrates. Hence, randomized use of 14C in glucose-6-phosphate is predicted for metabolism in order to complete the pentose cycle. 2) The significance in lack of accumulation of lactate in the experiment is that no abnormalities would be experienced during the pentose cycle. Accumulation in lactate would lead to metabolic dysregulation which would result in reduced or abnormal metabolism of pentose-phosphate 2. Metabolism would affect the pH balance in pentose-phosphate metabolism if lactate would be produced in high or excess amounts. The other explanation would be the incorporation of the lactate-acid cycle. Since the process is anaerobic the use of CO2 to convert the lactate reduced the amount of carbon (IV) oxide. Nonetheless, as glycolysis is happening probably the lactate acid cycle is taking place. Therefore, based on the results of the experiment, the equilibrium in CO2 and O2 at 50% reduced the production of lactate.1Katz, J., Wood, H.G. “The use of glucose-C14 for the evaluation of the pathways of glucose metabolism.” J. Biol. Chem. 235, 2165–2177, 20102Hoff, J., Støren, Ø., Finstad, A., Wang, E., Helgerud, J. “Increased blood lactate leveldeteriorates running economy in world class endurance athletes.” J. Strength Cond. Res. 30, 1373–1378, 2016.
Student No./100033) The enzymes necessary for metabolic activity may have been depleted or were less compared to the number of available enzymes were lower than substrates 3. Therefore, the rate of oxygen input in addition carbon (IV) oxide output may have reached an equilibrium. The isomerization of 14C in substrates as a result of reduction of substrates and enzymes necessary for glycolysis. Another reason is that substrates may have reduced in amount whichmay have resulted in reduced metabolic activity leading to an equilibrium in oxygen input and carbon (IV) oxide output. 4) The opinion is that glycoside beta drummin may be an inhibitor to the enzymatic activityin glycolytic metabolism4. Glycolysis heavily relies on enzymatic action which is crucial to the catalysis of the cycle resulting into the creation of carbon (IV) oxide and intake of oxygen. As such, inability of the enzymes to induce metabolism, there was a reduced oxygen intake and carbon (IV) oxide output. The possible explanation is that the binding of the inhibitor, in this case, glycoside beta drummin, to the substrates, at concentration between 10-100mM created an imbalance in the enzyme-substrate complex as a result reducing metabolism.5) The addition of inhibition to RSW did not alter any results and thus indicates that glycoside beta drummin, can cause inhibition reaction in the glycolysis process. The plausibleexplanation is that glycoside beta drummin contains inhibitor molecules that either bind to the substrate-enzyme complex or on the enzymes limiting their biochemical functionality 5. 3Canto, C., Menzies, K.J., Auwerx, J. “NAD+ metabolism and the control of energy homeostasis: a balancing act between mitochondria and the nucleus.” Cell Metab. 22, 31–53, 2015. 4Vander Heiden, M.G., DeBerardinis, R.J. “Understanding the intersections between metabolism and cancer biology.” Cell 168, 657–669, 2017. 5Vander Heiden, M.G., DeBerardinis, R.J. “Understanding the intersections between metabolism and cancer biology.”Cell 168, 657–669, 2017.
End of preview
Want to access all the pages? Upload your documents or become a member.
Related Documents
Assign. 1: Question 1 Surname, First Name. a).Glucose mlg...
|4
|2931
|28
Chemistry and Biochemistry – BIOB111lg...
|11
|1544
|260
Exercise Physiology Assignmentlg...
|6
|1798
|98
CM 2 Carbohydrate Metabolismlg...
|12
|3301
|11
Biotechnology | Electron Transport Phosphorylationlg...
|7
|1532
|17
Four Stages of Respiration: Annotated Diagramlg...
|5
|1092
|66