Report on Bioassay Lab 2022

Running head: LAB REPORT
BIOASSAY LAB REPORT
Name of the Student
Name of the University
Author note

LAB REPORT1
Introduction
A bioassay is defined as an experiment that helps in the determination of the potency as well
as concentration of the compound my calculating the biological responses. The bioassays
measures drug concentration, drug potency and calculate pharmacological activity of other
drugs (Stypuła-Trębas et al. 2017). The necessary requirements of bioassays. In the following
practical, bioassays are done to quantify drug concentration in biological activity. Biological
substances activities of an unknown are compared with international standard and the results
are expressed as clotting factor, hormones or other mediator (Cutignano et al. 2015)
Methods
The isolation of guinea-pig ileum was prepared like previous experiment. Two assays
followed are
Single point assay:
Increased concentration of 10μl of 10μg/ml ACh of standard ACh was added to tissues till
maximum response was reached to plot log dose-response curve. The linear part of the curve
was noticed and estimated 50% of maximum response. Unknown was added to the organ
bath so that to obtain a volume that stimulates the 50% of responses and (VU) was read the
exact response (RU) to the bulk from the curve as well as the organ bath concentration was
obtained from log dose dependant response for the standard that resembles to the reaction.
Unknown of ‘Y’ ml produced a response of R, then the organ bath concentration would be B
(ng/ml). The ‘X’ ml of unknown have (B x organ bath) ng. Hence, the concentration of
unknown obtained was (B x organ bath vol)/Y) ng/ml. The following formula was used in
determining the stock concentration of unknown
Organ bath vol. (ml) X Concentration of unknown read off (ng/ml)
Vol. of unknown added (ml)

LAB REPORT2

LAB REPORT3
Bracketing assay:
Two concentration were selected for standard which comes under the linear portion of the log
concentration-response curve, among 30% plus 70% of concentrated response towards the
standard (S1 and S2). The volume of the unknown was determined that produces a reaction
roughly midway amongst the above two standard responses (U). The responses were repeated
as per the bracketing assay such as S1-U-S2-U-S1-U-S2-U-S1. Each of the ‘brackets’ were
plotted on distinct log concentration standard-response graphs. The calculation for the
original concentration of the unknown by the technique designated for single point assay
were performed. Four estimations were provided for the concentration of the original
unknown solution shown in μg/ml or ng/ml. The mean ±standard deviation was obtained for
the value received. The concentration was converted to Molar concentration, such as (g/L)/
MWt (Molar weight of Ach = 181.6).
Result
Two concentrations such as 1.114 and 1.1431 of the standard falls in the linear portion of the
log concentration-response curve, among 30% and 70% of maximum response produced to
the S1 and S2 standard. A 40ul volume of unknown produced a response that is
approximately halfway between the two standard responses (U). Table for acetylcholine
graph Log Conc. Ng/ml 0.477 0.845 1.114 1.431 1.724 2.068. Response 0.1589 0.2351
0.2874 0.762 0.8011 0.8926. etc.
Trace of result
The result obtained suggested that Unknown result U1 and U2 have a response rate between
20% to 80% and by doing graphical analysis it was obtained that U1 have a response of 0.60
and 0.40 respectively. All the data interpretation was done from the data obtained. The
activity of unknown sample is between two known samples.