Mayo Clinic is a non-profit healthcare organization that provides wide-ranging healthcare facilities and programs related to healthcare education, investigation, and other patient care services. This article provides a SWOT analysis of Mayo Clinic, analyzing its strengths, weaknesses, opportunities, and threats. The strengths of Mayo Clinic include effective clinical services through research, innovation, and education, efficient teams involved in multidisciplinary inquiry, research and innovation, and emphasis on quality. The weaknesses of Mayo Clinic include lack of fixed payment system and lack of diversity synergy while dealing with patients of diverse cultures. The opportunities of Mayo Clinic include significant expansion in technical abilities to attain organizational growth, highly equipped healthcare and services to severely critical patients, use of specialized treatment and services to cater aged patients, and affiliation with other hospitals to expand its services to reach into new states and develop a larger organization. The threats of Mayo Clinic include tough competition from other prominent healthcare organizations in the United States and lack of qualified medical practitioners in the USA.