Biochemistry, Preservation and Processing of Meat
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This article discusses the biochemistry of meat, its composition, and the various methods of meat processing and preservation. It covers topics such as curing, cutting/grinding/mincing, smoking, and freezing/chilling. The article also highlights the dangers of freezing or chilling meat and the benefits of smoking meat. Course code, course name, and college/university are not mentioned.
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Running head: BIOCHEMISTRY, PRESERVATION AND PROCESSING OF MEAT 1
Biochemistry, Preservation and Processing of Meat
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Biochemistry, Preservation and Processing of Meat
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BIOCHEMISTRY, PRESERVATION AND PROCESSING OF MEAT 2
Meat Biochemistry
Meat is obtained from the flesh of animals and used as food. It contains various
constituents such as water, fat, and protein. It is composed of connective tissue, muscle fibers,
adipose tissues, bones, and water. Connective tissue contains mucopolysaccharides and proteins.
It provides support to muscles by creating adhesion between the cells in the muscles. The major
proteins in connective tissues are reticulin, elastin, and collagen (Pearson, 2012). Adipose tissue,
on the other hand, is a layer of fat. The major fats are marbling and cover fats. Cover fat is
located in the cavity of the abdomen and gives insulation to the skin. Marble fat is found within
the muscles and has perceived texture, juiciness, and flavor.
Meat is obtained from poultry and mammals. In mammals, there is pork from pigs, beef
from cattle and veal from calves. Chicken, geese, turkey, duck, and quails. Meat is nutritious to
our body since it gives phosphorus, iron, thiamin, vitamin b16, riboflavin, zinc, and niacin. It
contains 18% protein, 75% water, 1.5% minerals, 3% fat and 1% glycogen. However, some of
these constituents differ with body size, age, and genetics of the animal producing the meat.
Water content for older animals is less. Beef has high levels of water as compared to goat, sheep,
and pigs (Toldrá, 2007).
Proteins from the meat give toughness and shape to muscles. Pigments obtained from
proteins gives the meat color. Blood and cell plasma are derived from proteins. Proteins from
meat also help in the growth of hair, horn, and nails. Amino acids in meat are plenty in meat than
in plants. The major amino acids composed in meat are valine, lysine, threonine, methionine,
isoleucine, leucine and phenylalanine. The quality in meat decreases with an increase in the
connective tissue (Toldrá, 2007).
Meat Biochemistry
Meat is obtained from the flesh of animals and used as food. It contains various
constituents such as water, fat, and protein. It is composed of connective tissue, muscle fibers,
adipose tissues, bones, and water. Connective tissue contains mucopolysaccharides and proteins.
It provides support to muscles by creating adhesion between the cells in the muscles. The major
proteins in connective tissues are reticulin, elastin, and collagen (Pearson, 2012). Adipose tissue,
on the other hand, is a layer of fat. The major fats are marbling and cover fats. Cover fat is
located in the cavity of the abdomen and gives insulation to the skin. Marble fat is found within
the muscles and has perceived texture, juiciness, and flavor.
Meat is obtained from poultry and mammals. In mammals, there is pork from pigs, beef
from cattle and veal from calves. Chicken, geese, turkey, duck, and quails. Meat is nutritious to
our body since it gives phosphorus, iron, thiamin, vitamin b16, riboflavin, zinc, and niacin. It
contains 18% protein, 75% water, 1.5% minerals, 3% fat and 1% glycogen. However, some of
these constituents differ with body size, age, and genetics of the animal producing the meat.
Water content for older animals is less. Beef has high levels of water as compared to goat, sheep,
and pigs (Toldrá, 2007).
Proteins from the meat give toughness and shape to muscles. Pigments obtained from
proteins gives the meat color. Blood and cell plasma are derived from proteins. Proteins from
meat also help in the growth of hair, horn, and nails. Amino acids in meat are plenty in meat than
in plants. The major amino acids composed in meat are valine, lysine, threonine, methionine,
isoleucine, leucine and phenylalanine. The quality in meat decreases with an increase in the
connective tissue (Toldrá, 2007).
BIOCHEMISTRY, PRESERVATION AND PROCESSING OF MEAT 3
Meat Processing
Previously processing was done on meat to preserve it. It is also a means of adding
variety from the change in flavor and texture created. Processing also increases the supply of
meat since a carcass can be mixed with lean meat. Again, some foodstuffs can be added on meat
like cereals and supplement the shortage. There are various procedures of processing meat
including heat treatment, mincing, curing, salting, grinding and adding seasonings
("PRINCIPLES OF MEAT PROCESSING TECHNOLOGY," n.d.).
Curing
This is the process of adding curing agent to meat such as sodium nitrite present in
common salt. This agent introduces an attractive red or pink color which increases consumers'
desire. This is a major processing done for sausages, bacon, and ham which makes them heat
stable. However, nitrite is only applied in small amounts in coloring and meat preservation. To
prevent overdose, a mix proportion is prepared by manufacturers which limits the amount to
0.5%. With sodium chloride and nitrite in this proportion, the latter is called nitrite curing salt.
This mixture is added at a level between 1.5-3% to achieve the required flavor ("List of
the Best Meat Preservation Methods," 2018).
While applying this mix, contact is kept on myoglobin and the muscle tissue. This mixture also
increases the pH of acidified meat fresh from slaughter. Nitrite interacts with myoglobin to form
a heat-stable compound known as nitrosomyoglobin. At quantities of below 2%, nitrite is less
toxic to consumers. Adding nitrite in the curing process is beneficial in a number of ways
including:
It makes fat stable retards rancidity in meat
Enhances the flavor in meat after curing
Meat Processing
Previously processing was done on meat to preserve it. It is also a means of adding
variety from the change in flavor and texture created. Processing also increases the supply of
meat since a carcass can be mixed with lean meat. Again, some foodstuffs can be added on meat
like cereals and supplement the shortage. There are various procedures of processing meat
including heat treatment, mincing, curing, salting, grinding and adding seasonings
("PRINCIPLES OF MEAT PROCESSING TECHNOLOGY," n.d.).
Curing
This is the process of adding curing agent to meat such as sodium nitrite present in
common salt. This agent introduces an attractive red or pink color which increases consumers'
desire. This is a major processing done for sausages, bacon, and ham which makes them heat
stable. However, nitrite is only applied in small amounts in coloring and meat preservation. To
prevent overdose, a mix proportion is prepared by manufacturers which limits the amount to
0.5%. With sodium chloride and nitrite in this proportion, the latter is called nitrite curing salt.
This mixture is added at a level between 1.5-3% to achieve the required flavor ("List of
the Best Meat Preservation Methods," 2018).
While applying this mix, contact is kept on myoglobin and the muscle tissue. This mixture also
increases the pH of acidified meat fresh from slaughter. Nitrite interacts with myoglobin to form
a heat-stable compound known as nitrosomyoglobin. At quantities of below 2%, nitrite is less
toxic to consumers. Adding nitrite in the curing process is beneficial in a number of ways
including:
It makes fat stable retards rancidity in meat
Enhances the flavor in meat after curing
BIOCHEMISTRY, PRESERVATION AND PROCESSING OF MEAT 4
Inhibits the growth of microorganisms commonly in the canned meat where heat-resistant
bacteria may have made way in the cans.
Increases the demand to the consumer by introducing an appealing red or pink color.
Cutting/ Grinding/Mincing
These are the mechanical processes of reducing the sizes of animal tissues. Mincers and
grinders are designed to cut or separate bones and hard tendons from soft meat tissues. Others
are bowl cutters which chop and mix fat, lean meat or frozen meat. Ingredients such as extenders
(binders/fillers), functioning additives (curing agents and salt) are added during the grinding and
mincing processes. Emulsifying machines first allows mixing with seasonings and additives to
before passing it in colloid mills to produce desirable sizes and emulsification in meat mixes.
Frozen meat is cut into slices using bowl cutters without thawing to prevent discoloration,
bacterial growth, and drip losses. For small-scale processes, frozen meat cutting can be done
using axes and cleavers. Fatty tissues are cut into cubes of 2-4cm to aid in chopping in
emulsifiers and cutters. It can also be done for small-scale processing (Heinz, & Hautzinger,
2007).
Smoking
This is a process of dressing meat products with smoke from raw wood. Smoke is created
when cellulose and lignin are destroyed by thermal destruction from heat. Thermal action on
wood dissociates about 1000 desirable and undesirable components in wood (Dierenfeld, 2015).
These components are beneficial for processed meat in the following ways:
Adds a smoking taste
Fat oxidation retardation
Aldehydes and phenols reduce the antioxidant impact
Inhibits the growth of microorganisms commonly in the canned meat where heat-resistant
bacteria may have made way in the cans.
Increases the demand to the consumer by introducing an appealing red or pink color.
Cutting/ Grinding/Mincing
These are the mechanical processes of reducing the sizes of animal tissues. Mincers and
grinders are designed to cut or separate bones and hard tendons from soft meat tissues. Others
are bowl cutters which chop and mix fat, lean meat or frozen meat. Ingredients such as extenders
(binders/fillers), functioning additives (curing agents and salt) are added during the grinding and
mincing processes. Emulsifying machines first allows mixing with seasonings and additives to
before passing it in colloid mills to produce desirable sizes and emulsification in meat mixes.
Frozen meat is cut into slices using bowl cutters without thawing to prevent discoloration,
bacterial growth, and drip losses. For small-scale processes, frozen meat cutting can be done
using axes and cleavers. Fatty tissues are cut into cubes of 2-4cm to aid in chopping in
emulsifiers and cutters. It can also be done for small-scale processing (Heinz, & Hautzinger,
2007).
Smoking
This is a process of dressing meat products with smoke from raw wood. Smoke is created
when cellulose and lignin are destroyed by thermal destruction from heat. Thermal action on
wood dissociates about 1000 desirable and undesirable components in wood (Dierenfeld, 2015).
These components are beneficial for processed meat in the following ways:
Adds a smoking taste
Fat oxidation retardation
Aldehydes and phenols reduce the antioxidant impact
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BIOCHEMISTRY, PRESERVATION AND PROCESSING OF MEAT 5
Prevents microbial activities
Aldehydes and carbonyls form an attractive smoke color
Aldehydes cause hardening on the surface of casings or sausages
Phenols, acids, and aldehydes preserve meat.
However, smoking is toxic in some levels from the benzopyrene residues which are
carcinogenic. The carcinogenic level from smoking depends on the longevity of smoke
application. Smoking is thus regulated at varying temperatures. Hot and cold smoking are the
two types of smoking processes in meat. For both methods, smoke is allowed to diffuse into
outer layers of meat to enhance preservation, color, and flavor.
Cold smoking is a common process that applied in the olden days to preserve meat.
Today it is used to enhance color and flavor formation. An example is smoking blood sausages
and liver sausages made out of precooked foodstuffs. Temperature is achieved at 15 to 26
degrees centigrade for cold smoking. Meat is hung away from the slow-burning sawdust smoke.
Cold smoking consumes a lot of time and is repeated after a few days in a period of few hours
each day.
Hot smoking, on the other hand, is conducted on a range of temperature of 60 and 80
degrees centigrade. Additional heat is applied in the heating chamber since smoke cannot attain
this temperature. This high temperature ensures rapid flavor and color development. Similarly,
heat is also regulated to prevent excess flavor and color formation. Sausages with a thin caliber
are smoked for 10 minutes while those with a thick caliber (ham, bacon, and bologna) are
smoked for up to one hour (Dierenfeld, 2015).
Prevents microbial activities
Aldehydes and carbonyls form an attractive smoke color
Aldehydes cause hardening on the surface of casings or sausages
Phenols, acids, and aldehydes preserve meat.
However, smoking is toxic in some levels from the benzopyrene residues which are
carcinogenic. The carcinogenic level from smoking depends on the longevity of smoke
application. Smoking is thus regulated at varying temperatures. Hot and cold smoking are the
two types of smoking processes in meat. For both methods, smoke is allowed to diffuse into
outer layers of meat to enhance preservation, color, and flavor.
Cold smoking is a common process that applied in the olden days to preserve meat.
Today it is used to enhance color and flavor formation. An example is smoking blood sausages
and liver sausages made out of precooked foodstuffs. Temperature is achieved at 15 to 26
degrees centigrade for cold smoking. Meat is hung away from the slow-burning sawdust smoke.
Cold smoking consumes a lot of time and is repeated after a few days in a period of few hours
each day.
Hot smoking, on the other hand, is conducted on a range of temperature of 60 and 80
degrees centigrade. Additional heat is applied in the heating chamber since smoke cannot attain
this temperature. This high temperature ensures rapid flavor and color development. Similarly,
heat is also regulated to prevent excess flavor and color formation. Sausages with a thin caliber
are smoked for 10 minutes while those with a thick caliber (ham, bacon, and bologna) are
smoked for up to one hour (Dierenfeld, 2015).
BIOCHEMISTRY, PRESERVATION AND PROCESSING OF MEAT 6
Meat Preservation
Meat products are extremely perishable and become hazardous for human consumption
from disintegration by endogenous enzymes, chemical change, and microbial growth. These
actions can be reduced by heating to kill enzymes and microorganisms, water removal by
osmotic control and drying, temperature reduction to prevent bacterial growth and chemicals use
to inhibit growth. Ancient methods of preservation include smoking, salting, sun and wind
drying. Canning introduced in the nineteenth century has been adopted to preserve meat for years
without destruction. Others include refrigeration, ionizing radiation, and smoking (Ercan &
Soysal, 2013).
Freezing and Chilling
Refrigeration was borrowed from the idea Romans used to preserve meat products. The
temperature for freezing and chilling is between 0° to 4°C. Similarly, packaging technics applied
include storing under a vacuum, nitrogen and carbon dioxide. This keeps the longevity of meat
products up to 10 weeks. Chilling is mostly done closer to the meat's freezing point of - 15-
degree centigrade to eradicate the effects of pathogens and development of harmful organisms.
However, some pathogens are only inhibited at this temperature, for example, the harmless
pseudomonas species found on the surface vacuum and chilled meat (ur Rahman, et al, 2018).
There are some dangers of freezing or chilling meat. For example, if the meat is put to
temperatures below 10 degrees centigrade before the pH falls below 7, contacting of muscle
fibers occurs causing the meat to be tough after cooking. Another problem which occurs is
exudation of tissue fluids from contraction of muscle tissue during thawing (Savanović & Grujić,
2017).
Smoking
Meat Preservation
Meat products are extremely perishable and become hazardous for human consumption
from disintegration by endogenous enzymes, chemical change, and microbial growth. These
actions can be reduced by heating to kill enzymes and microorganisms, water removal by
osmotic control and drying, temperature reduction to prevent bacterial growth and chemicals use
to inhibit growth. Ancient methods of preservation include smoking, salting, sun and wind
drying. Canning introduced in the nineteenth century has been adopted to preserve meat for years
without destruction. Others include refrigeration, ionizing radiation, and smoking (Ercan &
Soysal, 2013).
Freezing and Chilling
Refrigeration was borrowed from the idea Romans used to preserve meat products. The
temperature for freezing and chilling is between 0° to 4°C. Similarly, packaging technics applied
include storing under a vacuum, nitrogen and carbon dioxide. This keeps the longevity of meat
products up to 10 weeks. Chilling is mostly done closer to the meat's freezing point of - 15-
degree centigrade to eradicate the effects of pathogens and development of harmful organisms.
However, some pathogens are only inhibited at this temperature, for example, the harmless
pseudomonas species found on the surface vacuum and chilled meat (ur Rahman, et al, 2018).
There are some dangers of freezing or chilling meat. For example, if the meat is put to
temperatures below 10 degrees centigrade before the pH falls below 7, contacting of muscle
fibers occurs causing the meat to be tough after cooking. Another problem which occurs is
exudation of tissue fluids from contraction of muscle tissue during thawing (Savanović & Grujić,
2017).
Smoking
BIOCHEMISTRY, PRESERVATION AND PROCESSING OF MEAT 7
In the ancient times, meat was preserved by smoke from the wood fire. Nowadays there
are effective methods like the use of a smoke generator. Products from wood smoke enhance
appearances and flavor. For long storage, intensive smoking is encouraged which dries the meat
from hot air and deposition of enhancers on the meat surface. However, intense smoking creates
a detrimental effect on the color and flavor of the meat. The recent technology is to apply a
solution of smoke constituents which reduces the level of unwanted substances as well as flavor
("Meat and meat products in human nutrition ...," n.d.).
Massaging and Tumbling With Salt
This idea was introduced in 1960 to allow salt penetration in meat. Chopped meat is
injected with salt or immersed in the solution and then tumbled. A salt solution at 2 to 8 % are
used with sometimes polyphosphate used to extract myosin which is a water-soluble protein. The
reaction between the protein and the salt improves the water holding capacity in meat (Offer,
1988). The strong gel produced from the setting proteins bind the meat together to allow slicing
and shaping.
In the ancient times, meat was preserved by smoke from the wood fire. Nowadays there
are effective methods like the use of a smoke generator. Products from wood smoke enhance
appearances and flavor. For long storage, intensive smoking is encouraged which dries the meat
from hot air and deposition of enhancers on the meat surface. However, intense smoking creates
a detrimental effect on the color and flavor of the meat. The recent technology is to apply a
solution of smoke constituents which reduces the level of unwanted substances as well as flavor
("Meat and meat products in human nutrition ...," n.d.).
Massaging and Tumbling With Salt
This idea was introduced in 1960 to allow salt penetration in meat. Chopped meat is
injected with salt or immersed in the solution and then tumbled. A salt solution at 2 to 8 % are
used with sometimes polyphosphate used to extract myosin which is a water-soluble protein. The
reaction between the protein and the salt improves the water holding capacity in meat (Offer,
1988). The strong gel produced from the setting proteins bind the meat together to allow slicing
and shaping.
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References
A List of the Best Meat Preservation Methods - Countryside Network. (2018, June 26).
Retrieved from https://countrysidenetwork.com/daily/lifestyle/canning-food-
preservation/the-best-meat-preservation-methods/
Dierenfeld, J. D. (2015). Method for smoking meat. U.S. Patent Application No. 14/289,306.
Ercan, S. Ş., & Soysal, Ç. (2013). Use of ultrasound in food preservation. Natural Science, 5(08),
5.
Heinz, G., & Hautzinger, P. (2007). Meat processing technology for small to medium scale
producers.
Meat and meat products in human nutrition ... - Role of meat and meat products in human
nutrition. (n.d.). Retrieved from http://www.fao.org/docrep/t0562e/T0562E03.htm
Offer, G. (1988). The structural basis of water-holding in meat. Part I: general principles and
water uptake in meat processing. Developments in meat science.
Pearson, A. M. (Ed.). (2012). Muscle and meat biochemistry. Elsevier.
Potter, J. D. (2017). Red and processed meat, and human and planetary health. BMJ (Clinical
research ed.), 357, j2190.
PRINCIPLES OF MEAT PROCESSING TECHNOLOGY. (n.d.). Retrieved from
http://www.fao.org/docrep/010/ai407e/AI407E04.htm
Savanović, D., & Grujić, R. (2017). Protein changes during the freezing and storage of meat and
meat products. Veterinarski žurnal Republike Srpska, 17(1), 105-118.
Toldrá, F. (2007). Biochemistry of meat and fat. Handbook of fermented meat and poultry, 51-
58.
References
A List of the Best Meat Preservation Methods - Countryside Network. (2018, June 26).
Retrieved from https://countrysidenetwork.com/daily/lifestyle/canning-food-
preservation/the-best-meat-preservation-methods/
Dierenfeld, J. D. (2015). Method for smoking meat. U.S. Patent Application No. 14/289,306.
Ercan, S. Ş., & Soysal, Ç. (2013). Use of ultrasound in food preservation. Natural Science, 5(08),
5.
Heinz, G., & Hautzinger, P. (2007). Meat processing technology for small to medium scale
producers.
Meat and meat products in human nutrition ... - Role of meat and meat products in human
nutrition. (n.d.). Retrieved from http://www.fao.org/docrep/t0562e/T0562E03.htm
Offer, G. (1988). The structural basis of water-holding in meat. Part I: general principles and
water uptake in meat processing. Developments in meat science.
Pearson, A. M. (Ed.). (2012). Muscle and meat biochemistry. Elsevier.
Potter, J. D. (2017). Red and processed meat, and human and planetary health. BMJ (Clinical
research ed.), 357, j2190.
PRINCIPLES OF MEAT PROCESSING TECHNOLOGY. (n.d.). Retrieved from
http://www.fao.org/docrep/010/ai407e/AI407E04.htm
Savanović, D., & Grujić, R. (2017). Protein changes during the freezing and storage of meat and
meat products. Veterinarski žurnal Republike Srpska, 17(1), 105-118.
Toldrá, F. (2007). Biochemistry of meat and fat. Handbook of fermented meat and poultry, 51-
58.
BIOCHEMISTRY, PRESERVATION AND PROCESSING OF MEAT 9
ur Rahman, U., Sahar, A., Ishaq, A., Aadil, R. M., Zahoor, T., & Ahmad, M. H. (2018).
Advanced meat preservation methods: A mini review. Journal of Food Safety, e12467.
ur Rahman, U., Sahar, A., Ishaq, A., Aadil, R. M., Zahoor, T., & Ahmad, M. H. (2018).
Advanced meat preservation methods: A mini review. Journal of Food Safety, e12467.
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