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PY162 Therapeutics : Assignment
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Therapeutics (PY162)
Added on 2020-04-15
PY162 Therapeutics : Assignment
Therapeutics (PY162)
Added on 2020-04-15
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Running head: MICROBIOLOGICAL TECHNIQUESMICROBIOLOGICAL TECHNIQUESName of the StudentName of the UniversityAuthor Note
1MICROBIOLOGICAL TECHNIQUESContentsTask 1...............................................................................................................................................3Answer 1a....................................................................................................................................3Answer 1b....................................................................................................................................4Answer 1c....................................................................................................................................4Task 2...............................................................................................................................................5Answer 2a....................................................................................................................................5Answer 2b....................................................................................................................................5Answer 2c....................................................................................................................................6Task 3...............................................................................................................................................6Answer 3a....................................................................................................................................6Answer 3b....................................................................................................................................6Answer 3c....................................................................................................................................7Blood agar................................................................................................................................7Baird Parker agar.....................................................................................................................7Cetrimide agar.........................................................................................................................7MacConkey agar......................................................................................................................8Mannitol salt agar....................................................................................................................8XLD agar.................................................................................................................................8Answer 3d....................................................................................................................................9
2MICROBIOLOGICAL TECHNIQUESAnswer 3e....................................................................................................................................9Reference List................................................................................................................................10
3MICROBIOLOGICAL TECHNIQUESTask 1Answer 1aThe CFU/ml for the membrane filtration technique:The CFU/ml cannot be obtained for undiluted and solutions A and B because the cellscount were too numerous. Colony counts are usually made for counts between 25-250.For Solution D: 78 x 104 (multiplied by dilution factor). The CFU per ml will be (78 x 104)/9 = 8.7 x 104.Solution F gave rise to no colonies, so CFU/ml cannot be counted.The CFU/ml for the spread plate technique:The undiluted and solutions E and F cannot be used because for the undiluted solution,too many colonies were obtained (were above 250) and the other two solutions did not give riseto colonies.For solution B: 112 x 102 (multiplied by dilution factor). The CFU per ml will be (112 x 102)/0.2 = 56 x 103.Answer 1bThe plate counts are expressed as CFU/ml rather than cells/ml because dead or dyingbacteria will not form colonies, while CFU or colony forming units is a count of the number ofviable bacteria. Moreover, a colony may not have been formed by a single bacterium,particularly in the case of diplococci as they grow as a single colony (1).
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