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Microbiology Assignment: Bioscience

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Added on  2020-05-28

Microbiology Assignment: Bioscience

   Added on 2020-05-28

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Running head: BIOSCIENCEMicrobiology and Cell CultureName of the Student:Name of the University:Author Note:
Microbiology Assignment: Bioscience_1
BIOSCIENCE1Part 1The bacterial strains which will be used for differentiation through the selective mediaand microscopy are: Bacillus subtilis, Enterococcus faecalis, Staphylococcus epidermidis,Proteus mirabilis, Enterobacter cloacae, Escherichia coliBacillus subtilis- It is a catalase positive and gram positive bacteria which is found withinthe gastrointestinal tract of human and ruminants and also found in soil. The variousidentification tests which gives positive result are: Acid from glucose, acid from sucrose,catalase activity, endospore staining, gram stain. Bacillus subtilis grows in TSA plates and brothcultures.Under the light microscope, the flagella are clearly viewable with whip like log tails.Bacillus subtilis has peritrichous flagella. This means that the cell is entirely covered with smalltails. The tails are visible under a light microscope by staining with a specialized stain(Sciencedirect.com, 2018). Enterococcus faecalis- It is a gram positive bacterium that inhabits in the gastrointestinaltract of the mammals and other humans. The various identification test which gives a positiveresult are: gram staining, fermentation assays of glucose, sucrose, lactose are positive.Enterococcus faecalis grows in TSA plates, EMB plates, PEA plates, broth and slope cultures.This bacterium is closely related to the streptococci and under the microscope appear tobe cocci or spheres. It divides through binary fission and form chains of bacteria (Lebreton,Willems and Gilmore 2014). Staphylococcus epidermidis- It is a gram positive bacteria, which is a typically found onthe human skin flora and is not pathogenic. However, if a person has a weak immunity then there
Microbiology Assignment: Bioscience_2
BIOSCIENCE2is an increased chance of infection development. The various identification test which gives apositive result are: gram staining, hydrogen sulphide, fermentation assay of glucose, sucrose andlactose are positive. Staphylococcus epidermidis grows in TSA plate and broth, EMB plate, PEAplate.The colonies of Staphylococcus epidermidis under the microscopic view shows grape likeclusters, the colonies are cohesive and raised and measure about 1 to 2 mm in diameter (Namvaret al. 2014). Proteus mirabilis- It is an anaerobic bacterium that exhibits in soil and water. Thisbacterium is responsible for the majority of infection in humans. The various identification testwhich gives a positive result are: hydrogen sulphide test, fermentation assay of glucose ispositive. Proteus mirabilis grows in TSA plates and broth, PEA plate, EMB plate, SIM stabculture including use of Kovak’s reagent.The bacterium under the microscopic view is rod shaped with elongated flagella(Armbruster and Mobley2012).Enterobacter cloacae- The various identification test which gives a positive result are:fermentation assay of glucose and sucrose is positive. Enterobacter cloacae grows in TSAplates, SIM stab culture including use of Kovak’s reagent, broth and slant cultures.The microscopic view of the Enterobacter cloacae reveal straight rods, the coloniesrange from 1.2 to 3 micrometer, have 4 to 6 peritrichous flagella (Davin-Regli and Pagès 2015).Escherichia coli- The various identification test which gives a positive result are:fermentation assay of glucose and lactose is positive and is variable is sucrose. Enterobactercloacae grows in TSA plate and broth, PEA plate, EMB plate, SIM stab culture including use of
Microbiology Assignment: Bioscience_3

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