HPC1000 Human Nutrition: Diet Analysis of Mitochondrial Pyruvate

This report analyzes the impact of Mitochondrial Pyruvate Carrier 2 (MPC2) on hepatic gluconeogenesis through a detailed diet analysis. The study, involving liver-specific deletion of MPC2 in mice (LS-Mpc2-/-), reveals impairments in pyruvate metabolism and glucose production. The analysis highlights compensatory mechanisms like pyruvate-alanine cycling that mitigate the effects of MPC2 deficiency. Metabolomic analyses suggest altered amino acid metabolism contributes to residual glucose production. The report also discusses the effects of chemical inhibitors like UK-5099 and MSDC-0602 on pyruvate metabolism and glucose production, further emphasizing the role of MPC2 in controlling hepatic gluconeogenesis and unveiling compensatory pathways when MPC activity is inhibited.