This article discusses the methodology and techniques of molecular cloning, including preparation of LB medium, transformation of pFLAG, isolation and purification of pFLAG plasmid, digestion of pFLAG vectors, agarose gel electrophoresis, designing primers, polymerase chain reaction, ARFGEF3 touchdown PCR, and pGEM cloning. The article also includes a detailed flow chart and figures to illustrate the process.