Molecular Pathogenesis Assignment

Added on - 13 Apr 2020

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Running head: MOLECULAR PATHOGENESISMOLECULAR PATHOGENESISName of the StudentName of the UniversityAuthor Note
1MOLECULAR PATHOGENESISThe challenges associated with working with type 3 secretion systems ofC. violaceumisthat the genes clusters Cpi1, 1a and 2 are quite large (Batista & da Silva Neto, 2017). It is achallenge to identify the host targets of the effectors encoded by the type 3 secretion systems.Another challenge is the cross-contamination problems when using cell lines. The opportunitiesinclude the use of cell lines, learning the techniques of mutant construction and use of animalinfection models. This work will be connected with my personal expertise or interest as I haveknowledge about the molecular biology techniques that can be used for plasmid and mutantconstruction.The challenge will be to identify the proteins phosphoryalated by OspG (Zhouet al.,2013). OspG alters the host immune system by blocking the production of IL8. Mutations ofOspG will prevent the blocking of the host immune system, but will not be able to determine theproteins that are phosphoryalated by OspG. The opportunity will be to identify the proteins thatwere previously not known to be phosphoryalated by OspG. It has very little connection with myexpertise as it will involve the use of various bioinformatic softwares to determine the binding ofthe OspG to its necessary targets.The challenges involved are the presence of a large number of studies on this topic,IpaH mutants have no detectable phenotypes in cellular systems and the use of animal modelsare a cumbersome process (Wandelet al., 2017). The opportunity will be to identify the aminoacid residues of IpaH 9.8 that are involved in the binding and subsequent ubiquitinylation.However, it will help me to gain knowledge about new techniques like RNA interference,confocal microscopy, FACS, among others.
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