Ask a question from expert

Ask now

Paper- RNA-seq of tumor-educated platelets enables blood-based

1 Pages226 Words546 Views
   

Added on  2019-09-16

Paper- RNA-seq of tumor-educated platelets enables blood-based

   Added on 2019-09-16

BookmarkShareRelated Documents
Paper- RNA-seq of tumor-educated platelets enables blood-based pan-cancer, multiclass and molecular pathway cancer diagnostics”http://www.cell.com/action/showMethods?pii=S1535-6108%2815%2900349-9Data- https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE68086I would like to do a comparison of methods for analyzing the RNA-Seq data in Myron G.Best et.al’s paper . The paper focused on Tumor-educated-blood platelets and their role in tumor growth They sequences 283 platelets from 6 difference tumor types. The primary tumor was correctly identified 71% of the time. There results show that blood platelets can give an indication of where the cancer will be found and can provide diagnostic advances in oncology. The data was analyzed by using voom. This will be under the limma package that we have used. I want to compare the analysis with edgeR and DESeq2. I believe seeing the similarities and differences in the type of analysis will show how important methodology is I reproducing an experiment. Method:Normalize and perform QC of the DataedgeR differential expression analysis voom differential expression analysis and comparison to results in the paper.DESeq2 differential expression analysis. The last part is the comparison of the three methodologies. I will show similarities , differences and which method worked the best in finding more differentially expressed genes.
Paper- RNA-seq of tumor-educated platelets enables blood-based_1

End of preview

Want to access all the pages? Upload your documents or become a member.

Related Documents
RNA-Sequencing Data Analysis: A Comparison of mRNA Expression in Three Human Cell Types
|21
|2472
|463

Bioinformatics Assignment Sample
|5
|837
|24

DE Analysis App: Comparison of Count Data Analysis Methods
|13
|920
|439

Characterizing candidate genes for glyphosate resistance in Erigeron bonariensis
|2
|570
|448

Study on Detection of Breast Cancer
|4
|665
|193

Canine Melanoma Diagnosis in Dogs By Student Name Institute Name Course Name
|9
|4186
|106