The assignment content discusses various computer-related topics, including the Fetch-Decode-Execute cycle, von Neumann Architecture, Little-Man Computer, DOS and Linux commands, batch scripting, data structures (Queue, Stack, Heap), Job Scheduler, process statuses, Process Control Block, deadlock, file storage allocation schemas, and Access Control Matrix. The content also includes a programming question to write a program using the Little-Man Computer to calculate the sum of two numbers.