Principle of Disease Investigation in Medical Microbiology

Principle of disease
investigation in medical
microbiology

Contents
INTRODUCTION.....................................................................................................................................3
Material and method.............................................................................................................................3
Result......................................................................................................................................................4
DISCASION AND CONCLUTION.........................................................................................................6
REFRENCES..................................................................................................................................................7

INTRODUCTION
Microbiology is the biggest field of scientific study which is use for the study of microbes and the
features. Farther it divided into the small sab branches such as bacteriology, virology, immunology,
medial microbiology, all these field deal with study of types of microbes, their characteristics, their
impact on human immune system and health (Barer, M.R. and Irving, W.L., 2018). Medical microbiology it
is the biggest sub unit of microbiology which deals with the study of impact of every single type of
microbes, in human body system along with the development of medical tenement’s, medicines for every
type of microbial decease. Function of medical microbiology is to develop the prevention strategies,
diagnosis techniques, the majority of Microbes which causes the infectious disease are fungi, bacteria,
parasites and viruses. purpose of this report is to use the medical microbiological interpretation for
identifying the problem of case study Mr. John Smith of related to the urine tract infection if he has that
infection then what would be the particular causative microbe so that doctor can provide the appropriate
treatment related to that causative organisms.
Material and method
For the identification of UTI causing microbe we had used two methods plating technique and
API test strip. material required for the plating technique are (CLED) cysteine lactose electrolyte deficient
agar along with 10μl calibrated disposable inoculation loop (Leber, A.L., 2020). For plating inoculation, it
required labeled (CLED) plate with the name of group and date as well as patient name, then for initiating
the procedure it requires gently mix of the collected patient urine sample by avoiding the foaming. then
inoculated the plate by dipping a sterile inoculation loop into the sample, after the collecting the sample in
loop it followed by gentle streaking of the sample at the surface of agar plate. after the inoculation it
followed with the incubation of inoculated plate at 37C for 18 hours it can be incubate for overnight. after
the 18 hours’ incubation it proceed with the colonies counting by the use of colonies counter along with
the identification of medium colour around the colonies. for the identification of bacterial class, it
proceeds with the gram staining method, for this method it needs the preparation of air dried smear by the
use of sterile loop, 10μl water, clean glass slide. we placed one of the colonies at test tube for dilution
over the 10μl water then placed the drop of dilution on the clean slide. after the smear preparation it needs
the heat fixing and it did by passing the smear slide through Bunsen flame 5 times then. Farther it
proceeds with the crystal violet staining then it rinses with the water (Murray, P.R., 2017). Then it followed
by the gram’s iodine addition for 1 mint, after the addition of iodine it rinses with the alcohol, acetone for
decolonization. axes ethanol is riced with water then it observed under the microscope at 100 x with the
oil immersion. then result is noted down in the table 3. We identified the microorganism as gram negative