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BIOL 2P02 Introduction to Molecular Biology

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Introduction to Molecular Biology (BIOL 2P02)

   

Added on  2020-04-13

BIOL 2P02 Introduction to Molecular Biology

   

Introduction to Molecular Biology (BIOL 2P02)

   Added on 2020-04-13

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Running head: RECOMBINANT DNA TECHNOLOGYCourse Code:RECOMBINANT DNA TECHNOLOGYName of the Student:Name of the Partner:Name of TA:Lab Section:Report Submission Date:
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1RECOMBINANT DNA TECHNOLOGYAbstractThis study was aimed at creating a genomic DNA library. Such a library helps the researcher toidentify the desired genes. The desired gene can be expressed to obtain the protein product. Inthis study the Bacillus subtilis genomic DNA was used to create the genomic DNA library. Thegenomic and plasmid DNA pUC18 were isolated and their concentrations were determined. Thegenomic and plasmid DNA were then subjected to digestion, followed by ligation andtransformed into the Escherichia coli DH5α cells on media containing X-gal/IPTG. The bluecolonies observed lacked the presence of the insert, while the white colonies had the desiredinsert ligated into the pUC18 vector. The recombinant plasmids isolated from the recombinant orwhite clones were digested with the same restriction enzymes. Double digestion resulted in thegeneration of the desired vector and the insert. Thus, the creation of the genomic DNA librarywas successful.
BIOL 2P02 Introduction to Molecular Biology_2
2RECOMBINANT DNA TECHNOLOGYTable of ContentsIntroduction......................................................................................................................................3Results..............................................................................................................................................4Lab 3 results.................................................................................................................................4Lab 4 results.................................................................................................................................4Lab 5 results.................................................................................................................................5Discussion........................................................................................................................................7Reference List..................................................................................................................................9Appendix........................................................................................................................................11
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3RECOMBINANT DNA TECHNOLOGYIntroductionBacillus subtilis is a Gram-positive non-pathogenic bacillus involved in the formation ofheat resistant and dormant spores (Logan, Niall and Paul). It is the most characterizedmicroorganisms among the various Gram-positive bacteria present. It genetic material can easilybe subjected to manipulation and as a result widely used in genetic engineering. It is usedcommercially for the production of a variety of enzymes, vitamins, flavoring agents and in theproduction of industrial nucleotides (Capozzi et al.).Genomic DNA libraries carry the entire genomic DNA sequence of the organism. Thegenomic DNA library can be used to determine the whole genome sequence of an organismhelps to determine the phenotypes regulated by the genes, determination of mutations present inthe genome and in the production of proteins expressed from the respective genes forcommercial use. Genetic engineering requires the use of recombinant DNA technology in order to carryout genetic manipulation of an organism. Genetic engineering can also be used to generatemutations of genes, whose functions are not known (Gaj, Gersbach, and Barbas). Various typesof products like insulin, industrial enzymes and even the human growth hormone has beenproduced by the use of genetically modified organisms (GMOs). Genetically modified crops canalso be produced using this technique (Bawa and Anilakumar). The overall purpose of this reportis “to generate the genomic DNA library of B. subtilis and carrying out the necessary steps todetermine its efficacy”.
BIOL 2P02 Introduction to Molecular Biology_4

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