Study on Massons Trichrome (MT) Procedure
Added on 2020-05-01
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Background of the study Masson’s trichrome (MT) procedure originated from Claude L. Pierre Masson (1880-1959) and Histology prefers the mentioned three colour staining method. Various colours such as green or blue collagen, black cell nuclei and pink or light red cytoplasm are produced by most recipe. Introduction Staining is a method which is used in diagnosing and studying the abnormal cells morphology e.g.cancerous cells stressing on the tissues structural components (Gamble and Bancroft). A distinction between dissimilar structures in a specimen’s tissue is achieved through staining and its examination is allowed under a light microscope (Cook, 2006). Numerous specimen are diagnosed microscopicallyby the use of Haematoxylin and Eosin, haematoxylin helps in staining the nuclei whereas staining of extracellular materials and cytoplasm is done by the Eosin (Cook and Bancroft, 1995). This method mentioned is among the main special staining methods used in laboratories and it expresses collective tissues comprising of extracellular materials and cytoplasm by using three different sized dyes during staining[CITATION Ulr13 \p 178 \l 2057 ]. Masson's trichrome produces three different colours namely; cytoplasm and muscles which are stained red with Biebrich scarlet, collagen are stained by aniline light green or blue depending on which one was used. AimsTo use Masson's trichrome in the identification of fibroids in the uterine tissue section.To comprehend the mechanism used by special stains.MethodMasson’s Trichrome The tissue area surrounded by the paraffin and fixed formalin was rehydrated and deparaffinised through 100%, alcohol, 95% alcohol and 70% alcohol. The tissue section then washed in distilled water before staining it in Weigert's iron haematoxylin for 10 minutes [CITATION And14 \p 430 \l 2057 ].The distilled water was used again to wash the tissue section after staining it using Biebrich scarlet-acid fuchsine solution for 15 minutes or until collagen was not red[CITATION Ulr13 \p 67 \l 2057 ] then transferred straight to aniline blue solution without rinsing and stained for a period of 5-10minutes . After that, the distilled water was used to rinse the tissue section for a short time before differentiated in 1% acetic acid solution for a minute[CITATION Joh13 \p 78 \l 2057 ]. The washing took place again before a quick dehydration through 95% ethyl alcohol, which was also used to wipe-off Biebrich scarlet-acid fuchsine staining. Then the section was mounted for light microscope examination [CITATION Tas14 \p 132 \l 2057 ].
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