Copy Gel Image and Draw Bands

Task 1:
Make a copy of the image of a gel given below. You can then draw appropriate bands in each lane based
on the description of what is loaded in each lane (given below). You can draw these bands by hand on a
printed version of the image or add the bands to the image using Powerpoint or similar software. If you
do create a hand-drawn version remember to take a photo and then insert that photo into the
document you submit.
We understand that your diagram will not be able to portray the bands to their exact base-pair size, and
that in some cases you may not be able to calculate an exact size for your bands. Just do your best in
terms of positioning the bands in the lanes with respect to the standard markers, but please do not
panic if the bands are a couple of mm away from “perfect” in your diagram. LABEL each band with its
size (in bp) – we will tolerate a ±10 bp difference between your value and the true value.
 Lane 1 – pGEX6P2 with NO insert, digested with Btg I and Pst I
 Lane 2 – pGEX6P2 with the PCR product inserted in the correct orientation (the coding sequence
is in the same direction as the GST encoded by pGEX), digested with Btg I and Pst I
 Lane 3 – pGEX6P2 with the PCR product inserted in the incorrectorientation (the coding
sequence is in the opposite direction compared to the GST encoded by pGEX), digested with
Btg I and Pst I
 Lane 4: In this lane draw the fragments that would result from a PCR that uses all of the
necessary PCR components (including pGEX6P2 with no insert as template, dNTPs, Taq
polymerase, buffer, water) and ONLY the SCREEN-R primer (no SCREEN-F primer).
 Lane 5: In this lane draw the fragments that would result from a PCR that uses all of the
necessary PCR components (including pGEX6P2 with the PCR product inserted as template,
dNTPs, Taq polymerase, buffer, water) and BOTH the SCREEN-F and SCREEN-R primers.
* Note: all the sites are marked on the plasmid map.

Answer: