E.Coli Transformation: Green Fluorescent Protein Practical Report

This report details the transformation of E.coli bacteria using green fluorescent protein (GFP). The experiment involves introducing a new gene into E.coli to alter its characteristics, focusing on the green fluorescent protein contained in the plasmid reporter protein. The process includes sub-cloning the green fluorescent protein into the pQE30 expression vector and purifying it using immobilized metal affinity chromatography. The report covers the materials and procedures used, results obtained from various practicals including PCR assays and DNA analysis, and a discussion of the findings. The goal of the experiment was to understand recombinant DNA techniques, the function of the reporter gene, and sterile methods for handling bacteria.