Treatment of Clinical Signs and Symptoms through Identification and Isolation Mechanism of Suspected Bacteria

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Treatment of the clinical Signs and Symptoms through Identification and Isolation mechanism of suspected
bacteria

INTRODUCTION
A 56-year-old man who was actually catherized became pyrexic, showed rigors and hypotension just eight days
after his admission to an ICU with the injuries that resulted from the road accident. In order to facilitate the
process of the treatment, the blood samples were taken from his venous line then introduce into the incubation
bottles that were sent to the laboratory of the micro-biology where they were incubated using automated
incubation machine(Paul 2014). The samples were flanged positive the following day but the subsequent gram-
stain was actually found to be negative on testing. Coagulase test further narrowed the identification down as it
produced a negative result, meaning that patient was not infected with Staphylococcus aureus, but rather with
coagulase-negative bacteria. Staphylococcus Epidermidis.
Methods
Identification through phenotypic and biochemical means
There was use of the gram staining mechanism for both positive and negative results. The slide was flooded with
the gram stain 1 and then left for 1 minute. The stains were then washed off with the water and slide flooded with
the gram stain2 for another 1minute.It was washed in water then blot dry.Decolorization was carried out for
30minutes by flooding with95%alcohol.
It was executed as portrayed by scholars (Paul 2014) Next, microscopic organisms were refined on 2 unique kinds
of specific media - Baird Parker agar, which is specific for Staphylococci which separates microorganisms,
especially Staphylococci, in light of the deoxyribonuclease creation(Markey, Leonard, Archambault, Cullinane
and Maguire 2013). A short time later, catalase test was performed to research whether bubble arrangement
happens upon contact among microscopic organisms and hydrogen peroxide because of catalase chemical
creation(Paul 2014) As to coagulase testing, it was performed by latex agglutination on a card so as to set up
whether the microorganisms are creating a coagulase protein(Wood 2012).Living being distinguishing proof at an

organism categories level was accomplished with the assistance of Microbact 12S Staphylococcal Identification
System.
Identification by molecular means
DNA was separated by boiling and centrifuging the example and after that Polymerase Chain Reaction (PCR)
blend was set up so as to enhance DNA of enthusiasm for nucleotide succession correlation. The 0.8 kb PCR-
intensified 16S rRNA quality parts were utilized as a sequencing layout close by with 8FPL and 806R
preliminaries. PCR results were confirmed by electrophoresis, which isolates DNA pieces dependent on their size
(Wood 2012). Phosphate bunches in DNA spine are contrarily charged, along these lines DNA pieces are pulled in
to the positive charge and the littler they are, the quicker they move through agarose gel(Paul 2014).The DNA
sections in the example were tried against the DNA parts of known length (stepping stool). Afterward, the
acquired outcomes were utilized to distinguish the grouping root by utilizing Basic Local Alignment Search Tool.
Suggested Methods for treatments
So as to build up potential treatment for the patient, antimicrobial testing was performed. Anti-toxin rings were
put on the plates with microorganisms to screen the bacterial development hindrance by different anti-infection
agents. In addition, Vancomycin Minimum Inhibitory Concentration (MIC) was resolved so as to test for bacterial
weakness to Vancomycin and to set up the base focus at which microbes are repressed.
RESULTS AND DISCUSSION
Biochemical and Phenotypic identification means
While surveying state morphology, it was discovered that white-colored level bunched settlements have
developed on the blood agar. Refined on particular media brought about bacterial development on Baird-Parker
yet not on DNase agar, meaning the absence of DNase action (Paul 2014) The outcomes for particular