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Different Methods of Chromatography - Analysis

   

Added on  2021-01-01

6 Pages1312 Words475 Views
Undertake chromatographictechniques to identifycomponents in mixtures

Table of ContentsINTRODUCTION...........................................................................................................................3MAIN BODY...................................................................................................................................3Evaluate the chromatographic techniques used in relation to outcomes and suggestimprovements..............................................................................................................................3Analyse own chromatograms and relate the factors that affect separation of mixtures toquality of results..........................................................................................................................4Correctly use of Chromatographic techniques to produce chromatograms................................4Explain the use of chromatographic techniques to separate mixtures........................................5CONCLUSION................................................................................................................................5REFERENCES................................................................................................................................6

INTRODUCTIONChromatography refers to an effective method of separating components of solute ofmixture on the basis of relative amounts of each solute distributed between moving fluid streamknown as mobile phase and a contiguous stationary phase. It include that mobile phase mayeither liquid or gas whereas stationary phase can be a solid or liquid (Block, Durrum and Zweig,2016). The present report will focus on chromatographic techniques used in relation to outcomesand improvements. It will also include analysis of chromatograms and factors affectingseparation of mixtures along with correct use of chromatographic techniques. The use ofchromatographic techniques to separate mixtures is given below. MAIN BODYEvaluate the chromatographic techniques used in relation to outcomes and suggest improvementsThe chromatographic techniques can be described as method of separation of componentsof mixture through slow passage over or through a material that absorbs solutes differently. Itconsist various types of methods such as column chromatography, ion exchange, Gel-permeation (molecular sieve), affinity, paper chromatography, Thin layer– chromatography, gas–chromatography, Dye ligand, hydrophobic interaction, pseudo affinity and high pressure liquidchromatography. However, these methods are based on partition that are very effective inseparation and identification of small molecules including carbohydrates, amino acids and fattyacids. Moreover, it has been analysed that column chromatography is most frequently utilised inorder to purify biomolecules (Butnariu, 2014). In addition to this, ion exchange chromatographyis based on electrostatic interactions among charged proteins groups and solid support materialmatrix. At the other hand, Gel permeation include separation of molecules on basis of differentmolecular sizes and affinity chromatography is helpful for purification of antibodies, hormones,enzymes, specific protein and nucleic acids. In paper chromatography, a thick filter papercomprised support and water drops settle on its pores made up stationary liquid phase and mobile

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