Urine Analysis To Identfy Disease Report

Running head: URINE ANALYSIS TO IDENTFY DISEASE
URINE ANALYSIS TO IDENTFY DISEASE
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URINE ANALYSIS TO IDENTFY DISEASE
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Introduction
The kidney is a bean-shaped filter organ found in pairs in the side of the spine in
every human being. The primary role of the kidneys is to filter the blood from substances that
are toxic for the cell and oxidative end products and to extract and maintain water and
electrolytes by producing urine that is discharged into the ureter and urinary bladder.
Nephrons in the kidney play the chief part in producing urine (Sherwood 2015). The standard
components of urine are urea, chloride, potassium, sodium, creatinine, dissolved organics,
and inorganic ions. Urinalysis (UA), also referred to like routine and microscopy (R&M), is a
series of urine components test measures and mostly used in medical diagnostic techniques
to identify the components present in urine (Fox 2015).
Method:
The urinary glucose concentration detection test requires to perform Benedict's test.
The Benedicts test is done in a few steps. 5ml benedicts reagent are taken in a pipette, and
few drops of sample urine were added to it. The mixture was then heated for 5 to 10 minutes.
After that, the test tube is cooled by keeping them under running water. The change of the
solution color will indicate the concentration of glucose in the blood (Tawfik et al, 2019).
The urine proteins can be detected by performing the biuret test. The urine sample
was acidified, and precipitants were added to the sample. The sample was then centrifuged
and put in the analyzer for the estimation of protein—the system mix the NaOH and biuret
reagent. The test result is estimated in a colorimetric way (Kumar and Gill, 2018).
The bile salt of urine can be detected by the sulfur test. Some dry sulfur powder is
sprinkled on the surface of the fresh urine. If the bile salt is present in the urine, the sulfur
powder will be sink (Mohapatra et al, 2016).

URINE ANALYSIS TO IDENTFY DISEASE
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Result:
SampleTest Observation Result
(+ve or
–ve)
Inference Diagnosis
A
Benedict's
Test
The solution
remains pale blue
after heating
-ve No glucose
in the urine
No glycosuria
Biuret Test The solution turns
pale violet
+ve Presence
of protein in
the urine
Kidney
diseases
Sulfur Test The sulfur powder
remains on the
surface of the
sample
-ve No bile in
the urine
Liver
function is
normal
B Benedict's
Test
Solution turns
orange
+++
ve
1500-
2000mg/ dl
Presence of
glucose in
urine
Biuret Test No change in color -ve No protein
in
sample
Kidney
function is
normal