Virkon Disinfectant Assignment

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Added on 2020-04-15

Virkon Disinfectant Assignment

Added on 2020-04-15

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Microbiology1

Introduction:Peroxygenic acid is the component of Vikron disinfectant. Virkon disinfectant can be usedagaisnt multiple microorganisms like viruses, some fungi, and bacteria. Virkon acts bycarrying out oxidation of proteins and constituents of cell protoplasm. It leads to the enzymeinhibition and damage to the cell wall integrity. DISINFX D-125 is another multipurposedisinfectant which can kill approximately 135 microorganisms. Virkon has the ability to kill20 microorganisms. Both of these disinfectants can kill microorganisms with contact time of10 minutes. Constituents of Virkon are potassium peroxymonosulfate and sodium chloride-based disinfectant. Constituent of DISNFX D-125 is a quaternary ammonium disinfectant(Moslehifard et al., 2015). Virkon was experimentally compared with the sodiumhypochloride in dental casts contaminated with Staphylococcus aureus, Pseudomonasaeruginosa, Bacillus subtilis and Candida albicans. It was evident that 1 % Virkon wasrequired for killing S. aureus, P aeruginosa, and Candida albicans. 3 % Virkon soluion wasrequired for killing B. subtilis. 1 % Virkon solution was more effective as compared to the0.525 % sodium hypochlorite solution in killing S. aureus, P. aeruginosa, and C. albicans.However, there was no difference between 1 % Virkon and 0.525 % sodium hypochlorite inkilling B. subtilis. However based on low toxicity and good environmental compatibility,Virkon is considered as better as compared to the sodium hypochlorite (Moslehifard et al.,2015). Penicillin G (Benzyl penicillin) can be obtained from the Penicillium chrysogenum bythe fermentation process by growing on the supplemented Corn Steep Liquor. Citric acid canbe obtained by the fermentation of Aspergillus niger and yield of citric acid can be increasedby using carbohydrates rich mollases. Results: Kelsey Sykes test for Disinfectants A, B and C: Initially, three different disinfectants A (50 %), B (100 %) and C (50 %) were used against P.aeruginosa. All these disinfectants proved effective in reducing microbial growth. A (50 %and C (50 %) showed no microorganisms in all the three series of testing. B (100 %) showedno microorganisms series 1 and 3 tests, while in series 2, one tube exhibited positive growthfor microorganisms. 2

Table 1 : Dsinfectant A, B and C in Kelsey Sykes Test.1 2 3 4 5 6 7 8 Test no. Date Disinfectant concentration CFU/ml test organism Organism growth in recovery medium after specified time Result Series 1 Series 2 Series 3 1 A (50% ) 3.3x108- - - - Pass2 B (100%) 3.3x108 - - + - --- - Pass3 C ( 50% ) 3.3x108 - - - - - - PassKelsey Sykes test for range of disinfectants A, B and C: For the evaluation of optimum concentration of disinfectants, A, B and C to eradicate all themicroorganisms, these disinfectants were diluted. Disinfectant A (0.5 and 0.6 %) showedmicroorganisms survival in all the tubes. Disinfectant B (1.0 %) didn’t showedmicroorganisms survival in series 1, while it showed microorganisms survival in series 2 and3. Disinfectant B (1.2 %) exhibited no growth in all the tubes of series 1 and 3 tubes with nogrowth in series 2 and all tubes with growth in series 3. Disinfectant C (1.5 %) didn’t showedany microbial survival in series 1 and 2, however it showed microbial survival in 2 tubes inseries 3. Disinfectant C (1.8 %) is most effective among all dilutions because it demonstratedno microbial survival in all the three series. Table 2: Dilutions of disinfectant A, B and C in Kelsey Sykes Test.1 2 3 4 5 6 7 8 Test no. Date Disinfectant conc. Number of CFU/ml Organism growth in recovery medium after specified time Results Series 1 Series 2 Series 3 1 2 3 May 1 May 1 May 1 A(0.5 %) B(1.0%) C(1.5%) 4.8 × 108 4.8 × 108 4.8 × 108+++++ -- - - - -- - - - +++++ +++++ - - - - - +++++ +++++ - - ++ - FAIL FAIL PASS 3

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