Comprehensive Research Report: Animal Cloning and Its Impacts

This research report provides a comprehensive overview of animal cloning, delving into its scientific aspects, historical context, and ethical implications. It begins with an executive summary highlighting the significance of cloning research and its potential benefits, while also acknowledging the challenges and failures encountered. The report then explores the process of animal cloning, including the literature review, methodology, and discussion of various cloning techniques such as nuclear transfer. It examines the history of cloning, starting with early experiments and progressing to the cloning of Dolly the sheep. The report also addresses the ethical issues surrounding animal cloning, including concerns about animal welfare, potential health problems in clones, and the possibility of human cloning. Furthermore, the report discusses the different types of cloning, ethical considerations and the future scope of cloning research. The report concludes with a summary of the key findings and their implications. Finally, it provides a list of references used throughout the report.

Running Head: RESEARCH REPORT
Research Report on Animal Cloning
Name of the Student
Name of the University

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Executive Summary
Animal cloning is a subject of intense interest among biologists, scientists and common people
alike. While some researchers carry out the research process in order to find new ways to breed
clones of animals, others breed some specific clones for commercial benefit. However, this type
of cloning also involves hybridization of two nearby species (like wooly pig, iron age pig, geep
and others) who are more productive than the original species. However, as of now, most of the
experiments have failed and the researchers have generally failed to develop viable organisms by
cloning and hybridization. This research report is focused on the study and analysis animal
cloning and its effects on the society as well as the world.

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Table of Contents
1.0 Introduction................................................................................................................................3
2.0 Animal Cloning: A Detailed Research......................................................................................4
2.1 Literature Review..................................................................................................................4
2.2 Methodology..........................................................................................................................7
2.3 Discussion..............................................................................................................................7
2.4 Ethical Issues.......................................................................................................................10
3.0 Conclusion...............................................................................................................................12
References......................................................................................................................................13

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1.0 Introduction
Cloning is a biological technique that is used to create a new organism that is genetically
identical to another organism. The first idea of cloning was brought forward by German
Embryologist Hans Spemann in 1935. However, initially, only trees and plans were cloned
successfully. Animal cloning attempts were started much later around the 1980s when various
species like frogs, pigs and others were used to develop clones. The first successful clone of a
mammal was of a sheep (named Dolly) that was born in 1996 (Song et al., 2016). From that
point of time till now, many attempts have been made to clone various other species especially
critically endangered or extinct species so that they can be brought back into the world.
However, the cloned animals were born, most of them died early due to various organ
complications (lung problems in most of the cases). Moreover, it has been found that of all
cloning experiments conducted, only around 30% have been successful. With increasing research
on cloning, ideas have arisen regarding the possibility of cloning a human being (Bohrer &
Bordignon, 2016). However, human cloning has since been branded as illegal in order to protect
ethical and religious beliefs of many communities. Before this, human cloning experiments have
been conducted and some scientists were even successful in cloning human embryos. In recent
times, researchers have proposed to conduct cloning techniques for therapeutic purposes.
However, the ethical factor for such research is still under debate (as many consider it to be an
unsafe technology and may result in abuse of the subject) and such, no such implementation has
been done yet.

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In this research report, the subject of animal cloning has been researched in detail and its
ethical implications have been discussed. Furthermore, a literature review has been conducted in
order to gather some more information on the same topic.
2.0 Animal Cloning: A Detailed Research
2.1 Literature Review
According to Heinrichs (2017), cloning or agamic generation is a normal type of
reproduction that is mainly found in some plants and microorganisms. All plant organs can be
wellsprings of agamic propagation, yet stems are the most widely recognized ones. Agamic
multiplication incorporates maturing (jellyfish, corals and tapeworms), fracture (worms), and
parthenogenesis (a few fishes, bugs, frogs and reptiles). Over the ground stems (stolons) of
strawberry plants deliver new plants. Underground stems incorporate rhizomes, globules, corms
and tubers. Leaves in irises and underlying foundations of aspen are additionally wellsprings of
new plants. In animals the conceptive procedure is likewise broadened to the point that any
instrument we can envision has just been executed (Slack, 2014). The different types of agamic
multiplication exist together with hermaphroditism and indiscriminate outside and inner sexual
intercourse. However, the majority of the animals replicate through parthenogenesis just at
specific circumstances. Aphids utilize parthenogenesis in the spring when they wind up with
adequate sustenance. Parthenogenesis is faster than sexual proliferation and allows speedy abuse
of accessible assets (Nichols, 2016). In bumble bees, prepared eggs move toward becoming
females, while haploid unfertilised (parthenogenetic) eggs move toward becoming guys.
However, it ought to be noticed that abiogenetic multiplication of warm blooded animals isn't a
normally happening marvel in spite of the fact that in mammalian generation hereditarily

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indistinguishable people, known as monozygotic twins, do happen. As per Saliba et al. (2014),
these can however not be considered clones in this regard on the grounds that: (a) they are not
the consequence of agamic generation and (b) they share all their hereditary material where
misleadingly delivered clones just offer their center DNA, though the mitochondrial DNA varies.
Dynamic acceptance of agamic plant generation (utilizing joining and establishing) has been a
typical practice in agribusiness since early mankind's history. It has been utilized to breed and
hold especially attractive qualities, for example, development, flavor and protection. In this
manner, cloning is another wonder in the historical backdrop of human advancement of ranch
animal species (Dillen et al., 2013). The primary cloning investigates animals date from the
nineteenth century. In 1891 Hans Driesch isolated the blastomeres (cells framed in the main
phases of embryonic improvement) of a two-cell developing life of ocean urchin mechanically
by shaking them in seawater. For this situation, one might say that people have utilized the
normally happening procedure of generation of the coveted plant species. Be that as it may, with
regards to cultivate animals, abiogenetic proliferation in nature is on a par with non-existent.
They all imitate by joining the qualities of two individual life forms. The cells began to develop
freely and shaped two entire ocean urchins (Ni et al., 2014). After eleven years a similar test,
with comparative outcomes, was performed by Hans Spemann in a vertebrate (lizard) utilizing a
hair from his child kid to isolate the cells. In any case, the inaccessibility of a productive taking
care of framework and, all the more critically, absence of acknowledgment that mammalian
oocytes and preimplantation incipient organisms require entirely controlled temperature for
advancement hampered the use of the method to warm blooded creatures for just about 80 years
(Jin et al, 2017). In the long run the primary fruitful fetus part was performed in local animals
with the reason for quick augmentation of profitable people.

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A proficient animal cloning innovation would give numerous new chances to
domesticated animals agribusiness, human prescription, and animal protection. Atomic cloning
includes the generation of animals that are hereditarily indistinguishable to the contributor cells
utilized as a part of a strategy known as Nucleus Transfer (NT). A large number of the pregnancy
misfortunes identify with disappointment of the placenta to create and work accurately. Placental
brokenness may likewise have an unfavorable effect on postnatal wellbeing (Ogura, Inoue &
Wakayama, 2013). These peculiarities are most likely because of off base epigenetic
reconstructing of the contributor genome following NT, prompting improper examples of quality
articulation amid the improvement of clones. Nonetheless, at exhibit it is a wasteful procedure: in
dairy cattle, just around 6% of the developing lives exchanged to the conceptive tracts of
beneficiary bovines result in solid, longterm surviving clones (Cibelli et al., 2013). Of concern
are the high misfortunes all through incubation, amid birth and in the post-natal period through to
adulthood. While some physiological tests on surviving clones recommend typicality, different
reports show an assortment of post-natal clone-related variations from the norm. This
changeability in result may reflect species-particular or potentially cloning methodological
contrasts. Significantly, to date it gives the idea that these clone-related phenotypes are not
transmitted to posterity following sexual propagation. This demonstrates they speak to epigenetic
blunders, as opposed to hereditary mistakes, which are amended amid gametogenesis. While this
needs affirmation at the atomic level, it gives beginning trust in the principal use of NT in
horticulture, specifically, the creation of little quantities of cloned cows from hereditarily
recreated bulls, for normal mating, to successfully disperse hereditary pick up. Notwithstanding
the animal welfare worries with the innovation, the hidden wellbeing of the animals and the
significant impact on sustenance security are basic perspectives that expect examination to

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increase administrative and shopper acknowledgment (Fernandes et al., 2016). The fundamental
procedure was first created in creatures of land and water in the 1950s and was utilized to
explore atomic totipotency in separated cell populaces. In domesticated animals species,
undifferentiated embryonic blastomeres were first utilized effectively in sheep, dairy cattle and
pigs. In later circumstances, embryonic NT has been stretched out in mice to incorporate the
utilization of other undifferentiated cell sorts including embryonic foundational microorganisms
got from the internal cell mass of blastocysts. Future changes in animal cloning will to a great
extent emerge from a more noteworthy comprehension of the atomic systems of reinventing. The
creation of atomic clones is a multi-step process that basically produces a whole living being
from the atomic deoxyribonucleic acid (DNA) of a solitary benefactor cell utilizing the nuclear
transfer process (Yu et al., 2016). On the other hand, the utilization of more separated cell sorts
got from either developing lives, embryos or most essentially grown-up animals, as on account
of 'Dolly' the sheep, toppled a doctrine in science concerning atomic totipotency from grown-up
cells and has opened new open doors and headings in look into. This has been named substantial
cell NT to recognize it from embryonic NT.
2.2 Methodology
This research is mainly based on analytic study of existing works on animal cloning as
well as review of current happenings regarding animal cloning research works. The entire
research has been carried out with the help of extensive literature review that provided sufficient
information regarding the history of animal cloning as well as future scope of research.
Moreover, sources like research journals, newspapers and recent blogs have been used to find
recent news of animal cloning research and what the scientists are currently trying to achieve.

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2.3 Discussion
the likelihood of switching the procedure of cell separation and, subsequently, of utilizing
more created cells for atomic exchange had been portrayed in 1938 by Hans Spemann. He
proposed that one could exchange cores of morula organize incipient organisms (around 4 days
old) or "more established cores of different cells" into enucleated eggs. He called the thought "to
some degree phenomenal" as a result of the predictable specialized troubles. Abnormally, the
two researchers who in the end completed Spemann's phenomenal analysis had not by any means
found out about his proposition at the time. Utilizing frogs, Mohammed, Ma and Lei (2017)
evacuated the cores of beneficiary eggs and embedded a contributor core. At first, the contributor
cores were gotten from morula arrange incipient organisms. Later they originated from tadpoles
and from intestinal epithelium. The strategy brought about an extensive accomplishment in early
advancement of incipient organisms. Be that as it may, the more separated benefactor cells was,
the less achievement could be acquired in the propelled phase of improvement. The work was
proceeded by numerous researchers. By the by (albeit a few audits state generally), the full
formative cycle couldn't be finished: giver cells from tadpoles brought about completely created
frogs, and contributor cells from completely created frogs brought about tadpoles, yet no grown-
up frogs were cloned from grown-up frog cells. It might seem impossible to miss that researchers
did not endeavor to utilize grown-up physical cells as nucleic contributors in the mammalian
investigations performed in the vicinity of 1986 and 1997. According to Keefer (2015), despite
the fact that some prior productions had shown that refined cells of incipient organisms at a
propelled phase of advancement could be utilized as givers for atomic exchange, the introduction
of Dolly was required for wide acknowledgment that it was conceivable to clone a developed
animal by evacuating the core of a physical cell from a grown-up and embeddings it into an

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enucleated egg. The cloned posterity disorder is a continuum, in that lethality or anomalous
phenotypes may happen at any period of advancement, contingent on the level of dysregulation
of key qualities, probably because of principal blunders in epigenetic reconstructing. Indeed,
even evidently typical clones may have strange direction of numerous qualities that are
excessively unpretentious, making it impossible to bring about a conspicuous phenotype. There
has been much verbal confrontation about Dolly's abbreviated telomeres and the likelihood of
untimely maturing and early beginning of illness in clones. Telomeres are districts of DNA at the
finishes of chromosomes which continuously abbreviate after every cell division in most
substantial cell sorts. While Dolly may have created joint inflammation and was euthanised at a
generally youthful age on account of a virallyinduced lung tumor, this may have come about
because of her to a great extent indoor lodging and dealing with instead of the way that she was a
clone. Different examinations have been opposing concerning telomere length in clones, with
reports of rebuilding to ordinary in dairy cattle and mice and even examples of broadened
telomere lengths. The disclosure of a telomere length rebuilding process that happens amid early
embryogenesis seems in charge of this. Typical telomere lengths have even been accounted for
after rehashed recloning in mice and cows and particularly, in the spermatozoa of physical cell
cloned bulls and ensuing descendants. Along these lines, in cows and mice in any event, it gives
the idea that telomere disintegration by and large does not happen in clones and is thusly
improbable to cause the long haul wellbeing and decreased future concerns raised by numerous
current reports. The larger part of (male) mice cloned from youthful Sertoli cells kicked the
bucket after roughly 500 days, which was around half of the life expectancy in control mice. The
reasons for death were extreme pneumonia and hepatic disappointment. It stays to be resolved
whether this is a general wonder with clones, however it seems, by all accounts, to be both cell

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sort and genotype particular, with other cloned mice having evidently ordinary life expectancies.
The mouse demonstrate has the upside of a shorter age interim and natural life expectancy to
screen for these impacts. While it is empowering that a few examinations report ordinary
soundness of four year old cow-like clones, it is too soon to recognize if phenotypes with shorter
life expectancies will likewise happen among animals. In spite of the fact that an imperative
issue, regardless of the possibility that cloning were to abbreviate life expectancy, it might be of
little hugeness in farming. In business hamburger generation, for example, cows might be
butchered at target live weight inside two years, or in the dairy business the normal life
expectancy of a bovine in the group is just six years. In these cases, the profitable existence of
cultivated animals is considerably not as much as far as possible for the species. In any case,
considers demonstrate that amongst weaning and four years old, the yearly death rate in steers
cloned from physical cells is no less than 8%. This is in stamped differentiation to the
unimportant mortality experienced with the posterity of clones and the commonly acknowledged
mortality of 2% to 3% for every annum in traditional peaceful cultivating. In spite of the fact that
the purposes behind death among the clones are variable, and some possibly preventable, the
primary mortality factor past weaning is killing because of musculoskeletal variations from the
norm. This incorporates animals with seriously contracted flexor ligaments and those showing
unending faltering, especially in draining bovines. This accentuates the point that any
fundamental frailties in cloned animals may not be completely uncovered until the point when
the animals are worried in some way.

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2.4 Ethical Issues
There are a lot of ethical issues associated with animal cloning. As a result of these
issues, the animal cloning research has always faced obstacles. Some of the ethical issues with
animal cloning are as follows.
Animal Rights – During the cloning of some species like Pyrenean Ibex (that has been
extinct since 2000), the offspring was born successfully but died with a few days or months due
to defective lungs. Some other offsprings of other species also died due to other complications.
Even the first mammal clone Dolly the sheep died due to accelerated aging process. All these
issues have angered the animal activists as they have since demanded stopping of such cloning
research activities. According to their argument, cloning of animals is against the law of animal
rights and should be banned like human cloning has been banned. Some activists have even
termed animal cloning as a torture of the subject animal as well as the cloned animal (owing to
the extreme amount of pain most of the cloned animals had to undergo due to various organ
complications). Some activists have opined that as the animal cloning should be banned on the
same grounds as human cloning.
Human Cloning – Since the first successful cloning of a mammal was achieved,
scientists since have pondered over the idea of cloning human beings. Some scientists were even
successful in cloning human embryos using biological fertilization processes. However, human
rights activists raised some issues that render the human cloning process unethical. Some of the
points that defy the process of human cloning are as follows.
Torture: Human cloning is considered as torture of the subjects to some as they think
most of the subjects will be unwilling to donate their cells for cloning. Moreover, it is also

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general belief that no individual will allow researchers to build a new version of him / her with
the same looks and genetic characteristics.
Security: Although no human clone has been developed yet, it is general belief that if
such a clone is made, it will be exactly identical to the subject including genetic characteristics,
facial looks, thumb prints and other exclusive individual human characteristics. However, it is
feared that if the cloned individual becomes hostile and misuses the subject’s identity, there will
be no way to differentiate between the two individuals. Hence, there are always security issues
related to the human cloning process.
Religion: Another main obstacle faced by human cloning is the religious issue. Most
religions believe cloning of human is against the God’s will as God is the one and only creator of
life and human should not even try to take His place by these scientific experiments. Considering
all the different views and risks, human cloning is finally banned all over the world.
3.0 Conclusion
In this research report, the subject of animal cloning has been researched in detail and its
ethical implications have been discussed. Furthermore, a literature review has been conducted in
order to gather some more information on the same topic. Regardless of the possibility that
specialized challenges had been settled and the pregnancy rates enhanced, it would have
remained the case that an incipient organism must be part one to two times and in this way can
make at most two to four hereditarily indistinguishable kin through fake part. Every one of the
analyses depicted above utilized embryonic cells as hotspots for contributor cores. In spite of the
fact that an imperative issue, regardless of the possibility that cloning were to abbreviate life
expectancy, it might be of little hugeness in farming. In business hamburger generation, for

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example, cows might be butchered at target live weight inside two years, or in the dairy business
the normal life expectancy of a bovine in the group is just six years. In these cases, the profitable
existence of cultivated animals is considerably not as much as far as possible for the species. In
any case, considers demonstrate that amongst weaning and four years old, the yearly death rate
in steers cloned from physical cells is no less than 8%. This is in stamped differentiation to the
unimportant mortality experienced with the posterity of clones and the commonly acknowledged
mortality of 2% to 3% for every annum in traditional peaceful cultivating.

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References
Bohrer, R. C., & Bordignon, V. (2016). CLONING ANIMALS BY NUCLEAR
TRANSFER. Biotechnology of animal reproduction/editors, Marcelo Marcondes, 293.
Cibelli, J., Wilmut, I. S., Jaenisch, R., Gurdon, J., Lanza, R., West, M., & Campbell, K. H.
(Eds.). (2013). Principles of cloning. Academic press.
Dillen, K., Ferrari, E., Tillie, P., Philippidis, G., & Helaine, S. (2013). Contribution to the
economic impact assessment of policy options to regulate animal cloning for food
production with an economic simulation model.
Fernandes, C. C. L., Martins, L. T., Neto, S. G., Tavares, K. C. S., Aguiar, L. H., Calderón, C. E.
M., ... & Bertolini, L. R. (2016). Gene expression, oocyte quality and embryo production
by cloning in goats supplemented with different diets. Small Ruminant Research, 144,
255-262.
Heinrichs, B. (2017). Ethical Aspects of Animal Cloning for Food Supply Published Online:
2009-01-08| DOI: https://doi. org/10.1515/9783110196832.3. 367. Issues, 21.
Jin, K., Yu, X., Zhao, R., Wang, Y., Zuo, Q., Zhang, Y., & Li, B. (2017). Cloning of chicken
(Gallus gallus) CREPT gene and its expression in chicken DF-1 cells. Journal of
Agricultural Biotechnology, 25(2), 186-195.
Keefer, C. L. (2015). Artificial cloning of domestic animals. Proceedings of the National
Academy of Sciences, 112(29), 8874-8878.
Li, E. (2014). Ruchika Jingar ECS 188 March 12, 2014 The Ethics of Human Cloning. Ethics.

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Matoba, S., Liu, Y., Lu, F., Iwabuchi, K. A., Shen, L., Inoue, A., & Zhang, Y. (2014).
Embryonic development following somatic cell nuclear transfer impeded by persisting
histone methylation. Cell, 159(4), 884-895.
Mohammed, E. A., Ma, Z. Y., & Lei, Z. H. (2017). cDNA Cloning and Distribution of
Neuromedin U and its Receptor in the Rabbit. Journal of Veterinary Science & Medical
Diagnosis, 2017.
Ni, W., Qiao, J., Hu, S., Zhao, X., Regouski, M., Yang, M., ... & Chen, C. (2014). Efficient gene
knockout in goats using CRISPR/Cas9 system. PloS one, 9(9), e106718.
Nichols, A. (2016). Public opinion and policy on animal cloning in Spain.
Ogura, A., Inoue, K., & Wakayama, T. (2013). Recent advancements in cloning by somatic cell
nuclear transfer. Phil. Trans. R. Soc. B, 368(1609), 20110329.
Saliba, W. P., Bayão, H. X. S., Drumond, R. M., Alvim, M. T. T., Matos, S. P. M., Belisário, H.
L. R., ... & Mesquita, J. H. L. (2014). Efficiency of bovine commercial cloning. Animal
Reproduction, 11(3), 481-481.
Slack, C. (2014). Association for Molecular Pathology v. Myriad Genetics, Inc.: What Are the
Effects on Cloning Extinct Animals and Agriculture Now That CDNA Is
Patentable. Drake J. Agric. L., 19, 347.
Song, H., Li, H., Huang, M., Xu, D., Wang, Z., & Wang, F. (2016). Big animal cloning using
transgenic induced pluripotent stem cells: a case study of goat transgenic induced
pluripotent stem cells. Cellular Reprogramming (Formerly" Cloning and Stem
Cells"), 18(1), 37-47.

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Verlinden, H., Lismont, E., Marchal, E., Tobe, S., & Vanden Broeck, J. (2016). Molecular
cloning and characterisation of the allatotropin precursor and receptor in the desert locust.
Yu, M., Muteti, C., Ogugo, M., Ritchie, W. A., Raper, J., & Kemp, S. (2016). Cloning of the
African indigenous cattle breed Kenyan Boran. Animal genetics, 47(4), 510-511.