Bioassay Lab Report: Analysis of Drug Concentration using Bioassays

This lab report details a bioassay experiment using the isolated guinea pig ileum to determine the concentration of an unknown drug, specifically acetylcholine (ACh). The experiment employed two bioassay techniques: single point and bracketing assays. The methodology involved preparing the guinea pig ileum, constructing log dose-response curves, and calculating drug concentrations based on biological responses. Results include tables of responses against organ bath concentrations, log concentration curves, and calculations for unknown concentrations using both assay methods. The discussion highlights the accuracy of the results, potential sources of error, and the importance of bioassays in analyzing biological threats and evaluating substance quality. The report also includes calculations for drug organ bath concentrations and conversion to molar concentrations, along with relevant references.