This bioinformatics assignment focuses on analyzing a mutant cDNA sequence to determine its origin, the protein it codes for, and the impact of the mutation on protein function. The analysis involves aligning mutant and wild-type cDNA sequences to identify nucleotide differences and deduce the resulting amino acid substitution. The study also identifies the most closely related human protein homolog and examines the evolutionary conservation of NADH dehydrogenase subunit 3 across various species, culminating in the construction of a phylogenetic tree to assess evolutionary relationships. Discrepancies between expected and obtained protein sequences are discussed in the context of potential mutations and protein folding issues. The assignment leverages bioinformatics tools and databases to provide insights into the functional and evolutionary implications of the mutant cDNA sequence.